Azadeh Arabzadeh, Tammy-Claire Troy, Kursad Turksen

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Claudin Expression Modulations Reflect an Injury Response in the Murine Epidermis Azadeh Arabzadeh, Tammy-Claire Troy, Kursad Turksen Journal of Investigative Dermatology Volume 128, Issue 1, Pages 237-240 (January 2008) DOI: 10.1038/sj.jid.5700966 Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Morphological changes, epidermal hyperproliferation, and changes in the epidermal expression of suprabasal-associated Cldns in response to a single exposure to TPA. Backskin samples from CD-1 mice at (a) 4, (b) 12, (c) 24, (d) 48, (e) 72, and (f) 96hours post-TPA-application were compared with their acetone-treated counterparts (g) for histological changes (hematoxylin and eosin) and epidermal proliferation was confirmed through the increased localization of Ki67-positive cells by immunofluorescence. Immunolocalization of Cldn6, Cldn11, and Cldn18 revealed expanded epidermal expression compartments after a single exposure to TPA. In addition, there was a shift in Cldn localization from the membrane to the cytoplasm from 24 to 72hours. In the repairing epidermis 96hours after TPA treatment, membrane localization of the suprabasal-specific Cldns was evident with an overall reduction in the expression compartment. Journal of Investigative Dermatology 2008 128, 237-240DOI: (10.1038/sj.jid.5700966) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Cldn1 relocalization in response to a single application of TPA. (a) Immunofluorescence analyses revealed that in the acetone-treated epidermis, the mature localization of Cldn1 was maintained in the basal and suprabasal layers. Cldn1 localization was modulated as observed after (b) 4, (c) 6, (d) 12, (e) 24, (f and i) 48, (g) 72, and (h) 96hours in response to a single application of TPA. Cldn1 became increasingly cytoplasmic, especially in the lower strata of the epidermis where some nuclear association was observed from 12 to 72hours after treatment; nuclei are demarcated in (i) by co-immunolocalization with Lamin B. By 96hours, membrane association and localization in all strata was indicative of epidermal normalization. Co-immunolocalization demonstrates that although Cldn1 nuclear association is evident in the basal and immediate suprabasal layers (arrows), the nuclei in the upper strata are void (star). Please note that in (a-h) Cldn1 antigen is detected using FITC-conjugated goat anti-rabbit antibodies (green); in (i) Cldn1 antigen is detected using Alexa Fluor 594-conjugated donkey anti-rabbit antibodies (red) and Lamin B antigen is detected using Alexa Fluor 488-conjugated chicken anti-goat antibodies (green). Journal of Investigative Dermatology 2008 128, 237-240DOI: (10.1038/sj.jid.5700966) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions