Relationship between protein concentration and several parameters

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Relationship between protein concentration and several parameters Relationship between protein concentration and several parameters.A, peak area and the number of unique parent ions of peptides versus injection amount of HSA. The most abundant tryptic peptide of HSA, LCTVATLR, was used for peak area measurement. Relationship between protein concentration and several parameters.A, peak area and the number of unique parent ions of peptides versus injection amount of HSA. The most abundant tryptic peptide of HSA, LCTVATLR, was used for peak area measurement. B, numbers of peptides counted in three different ways versus injection amount of HSA. C, protein concentration versus PAI for 46 proteins in neuro2a cells. D, protein concentration versus the number of peptides divided by molecular weight of proteins for 46 proteins in neuro2a cells. E, relationship between protein concentration and emPAI for 46 proteins in neuro2a cells. F, absolute quantitation of 46 proteins in neuro2a cells using emPAI. QSTAR with faster scans (0.55 s for each MS/MS scan) was used for these experiments. Protein concentrations in neuro2a cells were measured by spiking synthetic peptides to neuro2a cells as described under “Materials and Methods.” arb.u, arbitrary units. Yasushi Ishihama et al. Mol Cell Proteomics 2005;4:1265-1272 The American Society for Biochemistry and Molecular Biology