Volume 64, Issue 1, Pages 239-241 (January 2016) Is single portal vein approach sufficient for hypothermic machine perfusion of DCD liver grafts?  Andrea Schlegel, Philipp Kron, Michelle L. De Oliveira, Pierre-Alain Clavien, Philipp Dutkowski  Journal of Hepatology  Volume 64, Issue 1, Pages 239-241 (January 2016) DOI: 10.1016/j.jhep.2015.09.015 Copyright © 2015 European Association for the Study of the Liver Terms and Conditions

Fig. 1 Perfusion quality of HOPE in DCD liver grafts. (A) Hypothermic oxygenated perfusion (HOPE) was performed with cold (10°C) UW Gluconate+Fluorescein (rat: 0.1g/50ml, pig & human 0.25g; concentration: 0.5g/5ml) in rat, pig, and discarded human DCD liver grafts through the portal vein (∗). Fluorescence under dark light confirmed complete perfusion of liver grafts within 5min by macroscopy and by histology. (B) Perfusion of DCD pig livers either through the portal vein alone (HOPE∗) or through the portal vein and the hepatic artery (DHOPE++) confirmed no difference in perfusion quality of both approaches as determined by CT-Angiography (UW gluconate+contrast agent: 16mg iodine/3l UW Gluconate). (C)The extrahepatic bile duct in discarded human DCD livers became stained by fluorescein during HOPE through the portal vein alone within 5min (∗). (D) Histologically, samples from the distal main bile duct confirmed the presence of fluorescein in the complete biliar duct wall (∗) after HOPE through the portal vein alone. (E) Extrahepatic bile duct with vascular supply by portal and arterial branches. Journal of Hepatology 2016 64, 239-241DOI: (10.1016/j.jhep.2015.09.015) Copyright © 2015 European Association for the Study of the Liver Terms and Conditions