Molecular epidemiology of enterovirus 71, coxsackievirus A16 and A6 associated with hand, foot and mouth disease in Spain M. Cabrerizo, D. Tarragó, C.

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Molecular epidemiology of enterovirus 71, coxsackievirus A16 and A6 associated with hand, foot and mouth disease in Spain M. Cabrerizo, D. Tarragó, C. Muñoz-Almagro, E. del Amo, M. Domínguez-Gil, J. M Eiros, I. López-Miragaya, C. Pérez, J. Reina, A. Otero, I. González, J.E. Echevarría, G. Trallero Clinical Microbiology and Infection Volume 20, Issue 3, Pages O150-O156 (March 2014) DOI: 10.1111/1469-0691.12361 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Distribution of the number of EV-positive samples from all syndromes analysed in the Enterovirus Unit of the CNM between January 2010 and December 2012 (right-axis) and prevalent types from HFMD/rash/fever cases studied (left-axis) by month. CV-A, coxsackievirus A; EV, enterovirus; EV-B, enterovirus B species. Clinical Microbiology and Infection 2014 20, O150-O156DOI: (10.1111/1469-0691.12361) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Distribution of EV types associated with HFMD alone, HFMD plus onychomadesis, atypical rash and febrile syndromes by year. CV-A, coxsackievirus A; EV, enterovirus; EV-B, enterovirus B species. Clinical Microbiology and Infection 2014 20, O150-O156DOI: (10.1111/1469-0691.12361) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Phylogenetic analysis of Spanish (a) CV-A6 and (b) EV-71 sequences and others available in GenBank in the 3′-VPI region of the genome. Spanish strains are indicated by black circles. Trees were constructed using the neighbour-joining method based on the maximum composite likelihood distances model. Bootstrap resampling was used to demonstrate the robustness of grouping; values >80% are shown. In the EV-71 tree, two old Spanish sequences from 1999 and 2002, respectively, were also included (black squares). The EV-71 classification into genogroups A, B and C and subgenogroups CI-C4 is indicated. Clinical Microbiology and Infection 2014 20, O150-O156DOI: (10.1111/1469-0691.12361) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions