Multiple Epidermal Connexins are Expressed in Different Keratinocyte Subpopulations Including Connexin 31 Wei-Li Di, Elizabeth L. Rugg, Irene M. Leigh,

Slides:

Advertisements

Similar presentations

The CXC Receptor 2 Is Overexpressed in Psoriatic Epidermis

Advertisements

Skin-Specific Expression of ank-393, a Novel Ankyrin-3 Splice Variant

Peroxiredoxin is Ubiquitously Expressed in Rat Skin: Isotype-Specific Expression in the Epidermis and Hair Follicle Jeong Eun Lee, Byung Dae Kwon, Jee-Bum.

BM-40(Osteonectin, SPARC) Is Expressed Both in the Epidermal and in the Dermal Compartment of Adult Human Skin Nicholas Hunzelmann, Martin Hafner, Sabine.

CtBP1 Overexpression in Keratinocytes Perturbs Skin Homeostasis

Basement Membrane Zone Remodeling During Appendageal Development in Human Fetal Skin. The Absence of Type VII Collagen is Associated with Gelatinase-A.

HKAP1.6 and hKAP1.7, Two Novel Human High Sulfur Keratin-Associated Proteins are Expressed in the Hair Follicle Cortex Yutaka Shimomura, Noriaki Aoki,

Transcription Factors C/EBPα, C/EBPβ, and CHOP (Gadd153) Expressed During the Differentiation Program of Keratinocytes In Vitro and In Vivo Edward V.

Overexpression of Serpin Squamous Cell Carcinoma Antigens in Psoriatic Skin Atsushi Takeda, Dousei Higuchi, Tadahito Takahashi, Masashi Ogo, Peter Baciu,

Differential Expression of a Novel Gene in Response to hsp27 and Cell Differentiation in Human Keratinocytes Mojgan Hell-Pourmojib, Peter Neuner, Robert.

Coexpression of Integrin αvβ3 and Matrix Metalloproteinase-2 (MMP-2) Coincides with MMP-2 Activation: Correlation with Melanoma Progression Uta B. Hofmann,

Expression of Neutrophil Gelatinase-Associated Lipocalin in Skin Epidermis Seong Jun Seo, Ji-Young Ahn, Chang-Kwun Hong, Eun-Young Seo, Kyung-Chae Kye,

Bulge- and Basal Layer-Specific Expression of Fibroblast Growth Factor-13 (FHF-2) in Mouse Skin Mitsuko Kawano, Satoshi Suzuki, Masashi Suzuki, Junko.

Expression of Multiple Cytochrome P450 Enzymes and Multidrug Resistance- Associated Transport Proteins in Human Skin Keratinocytes Jens M. Baron, Daniela.

Loss of EPC-1/PEDF Expression During Skin Aging In Vivo

The mRNA for Protease Nexin-1 is Expressed in Human Dermal Papilla Cells and its Level is Affected by Androgen Tadashige Sonoda, Yuji Asada, Sotaro Kurata,

Kinesin and Kinectin Can Associate with the Melanosomal Surface and Form a Link with Microtubules in Normal Human Melanocytes1 Garnet Vancoillie, Jo.

Marie-Thérèse Leccia Journal of Investigative Dermatology

Epidermal Label-Retaining Cells: Background and Recent Applications

Enhancing 1α-Hydroxylase Activity with the 25-Hydroxyvitamin D-1α-Hydroxylase Gene in Cultured Human Keratinocytes and Mouse Skin Tai C. Chen, Xue Hong.

Molecular Cloning and Expression of Human Keratinocyte Proline-Rich Protein (hKPRP), an Epidermal Marker Isolated from Calcium-Induced Differentiating.

Epidermal and Hair Follicle Progenitor Cells Express Melanoma-Associated Chondroitin Sulfate Proteoglycan Core Protein Lucy Ghali, Soon-Tee Wong, Nick.

Expression of Cholesterol Sulfotransferase (SULT2B1b) in Human Skin and Primary Cultures of Human Epidermal Keratinocytes Yuko Higashi, Hirotoshi Fuda,

Integrin β6-Deficient Mice Show Enhanced Keratinocyte Proliferation and Retarded Hair Follicle Regression after Depilation Yanshuang Xie, Kevin J. McElwee,

Expression of the Homeobox Gene, Barx2, in Wool Follicle Development

Protein C Inhibitor is Expressed in Keratinocytes of Human Skin

Localization of Rat FGF-5 Protein in Skin Macrophage-like Cells and FGF-5S Protein in Hair Follicle: Possible Involvement of twoFgf-5 Gene Products in.

The Vesicular Acetylcholine Transporter Is Present in Melanocytes and Keratinocytes in the Human Epidermis Souna M.A. Elwary, Bhaven Chavan, Karin U.

Expression of Matrilin-2 in Human Skin

Expression of Calcium-Binding S100 Proteins A4 and A6 in Regions of the Epithelial Sac Associated with the Onset of Hair Follicle Regeneration Mayumi.

Μ-Crystallin, Thyroid Hormone-binding Protein, is Expressed Abundantly in the Murine Inner Root Sheath Cells Noriaki Aoki, Kaoru Ito, Masaaki Ito Journal.

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

The Majority of Keratinocytes Incorporate Intradermally Injected Plasmid DNA Regardless of Size but Only a Small Proportion of Cells Can Express the Gene.

Expression of T-Cadherin in Basal Keratinocytes of Skin

Expression of Lipogenic Factors Galectin-12, Resistin, SREBP-1, and SCD in Human Sebaceous Glands and Cultured Sebocytes Wesley J. Harrison, Jonathan.

The Melanocortin 5 Receptor is Expressed in Human Sebaceous Glands and Rat Preputial Cells Diane Thiboutot, Aruntha Sivarajah, Kathryn Gilliland, Zhaoyuan.

Amy Falconer, Mohammed Ikram, Clare E. Bissett, Rino Cerio, Anthony G

Higher Levels of Steroidogenic Acute Regulatory Protein and Type I 3β-Hydroxysteroid Dehydrogenase in the Scalp of Men with Androgenetic Alopecia WenChieh.

Eric J. Stelnicki, László G. Kömüves, Angela O

Trangenic Misexpression of the Differentiation-Specific Desmocollin Isoform 1 in Basal Keratinocytes Frank Henkler, Molly Strom, Kathleen Mathers, Hayley.

Eric A.G. Blomme, Charles C. Capen, Thomas J. Rosol

The Epidermal Stem Cell Factor Is Over-Expressed in Lentigo Senilis: Implication for the Mechanism of Hyperpigmentation Hideko Hattori, Makoto Kawashima,

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

Sharon Grehan, Charles Allan, Elizabeth Tse, David Walker, John M

Protease-Activated Receptor 2, a Receptor Involved in Melanosome Transfer, is Upregulated in Human Skin by Ultraviolet Irradiation Glynis Scott, Cristina.

John E. Olerud, Marcia L. Usui, Deniz Seckin, Diane S. Chiu, Claire L

Keratinocytes Express the CD146 (Muc18/S-Endo) Antigen in Tissue Culture and During Inflammatory Skin Diseases1 Wolfgang Weninger, Michael Rendl, Michael.

Expression of μ-Opiate Receptor in Human Epidermis and Keratinocytes

Bikunin, a Serine Protease Inhibitor, is Present on the Cell Boundary of Epidermis Cui Chang-Yi, Yoshinori Aragane, Akira Maeda, Piao Yu-Lan, Masae Takahashi,

Role of Cytotoxic T Cells in Chronic Alopecia Areata

Expression of Perilipin A on the Surface of Lipid Droplets Increases along with the Differentiation of Hamster Sebocytes In Vivo and In Vitro Noriko.

I. Elisabeth Ekholm, Maria Brattsand, Torbjörn Egelrud

Molecular Cloning and Tissue Expression of the Murine Analog to Human Stratum Corneum Chymotryptic Enzyme Assar Bäckman, Lennart Hansson Journal of.

Ramine Parsa, Annie Yang, Frank McKeon, Howard Green

A Novel Gene Expressed in Human Keratinocytes with Long-Term In Vitro Growth Potential is Required for Cell Growth Laure Aurelian, Cynthia C. Smith,

Transcriptional Repression of miR-34 Family Contributes to p63-Mediated Cell Cycle Progression in Epidermal Cells Dario Antonini, Monia T. Russo, Laura.

Hair Cycle-Specific Immunolocalization of Retinoic Acid Synthesizing Enzymes Aldh1a2 and Aldh1a3 Indicate Complex Regulation Helen B. Everts, Lloyd E.

Expression of Mast Cell Growth Modulating and Chemotactic Factors and their Receptors in Human Cutaneous Scars Barbara Hermes Journal of Investigative.

Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes Karla Cauza, Gabriele Hinterhuber, Ruth Dingelmaier-Hovorka, Karin.

M2 Muscarinic Acetylcholine Receptor (mAchR) Subtype Is Present in Human Epidermal Keratinocytes In Situ and In Vitro S.M.A. Elwary, S. Hasse, K.U. Schallreuter

Expression of Opsin Molecule in Cultured Murine Melanocyte

Alterations of Glucosylceramide-β-Glucosidase Levels in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Sabine Pfister, Elisabeth.

Immunoprotective UVA (320–400 nm) Irradiation Upregulates Heme Oxygenase-1 in the Dermis and Epidermis of Hairless Mouse Skin Munif Allanson, Vivienne.

Autoantibodies in Vitiligo Patients Recognize Multiple Domains of the Melanin- Concentrating Hormone Receptor Raju V.S.R.K. Gottumukkala, Elizabeth A.

Gli1 Protein is Expressed in Basal Cell Carcinomas, Outer Root Sheath Keratinocytes and a Subpopulation of Mesenchymal Cells in Normal Human Skin Lucy.

Permeability Barrier Disruption Increases the Level of Serine Palmitoyltransferase in Human Epidermis Francesca Alessandrini, Dr., Heidrun Behrendt

Redistribution of LRIG Proteins in Psoriasis

Epidermal Interferon-γ Inducible Protein-10 (IP-10) and Monokine Induced by γ- Interferon (Mig) but not IL-8 mRNA Expression is Associated with Epidermotropism.

Effect of Healing on the Expression of Transforming Growth Factor βs and their Receptors in Chronic Venous Leg Ulcers Allison J. Cowin, Nicholas Hatzirodos,

Matrix Metalloproteinase Inhibitor BB-3103 Unlike the Serine Proteinase Inhibitor Aprotinin Abrogates Epidermal Healing of Human Skin Wounds Ex Vivo1

Presentation transcript:

Multiple Epidermal Connexins are Expressed in Different Keratinocyte Subpopulations Including Connexin 31 Wei-Li Di, Elizabeth L. Rugg, Irene M. Leigh, David P. Kelsell Journal of Investigative Dermatology Volume 117, Issue 4, Pages 958-964 (October 2001) DOI: 10.1046/j.0022-202x.2001.01468.x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 SDS-PAGE analysis for Cx31 antibody. Cx31 protein was translated in vitro (TNT system) or in vivo (HeLa cells) as described in Materials and Methods and subjected to SDS-PAGE: lane 1, 14C-labeled molecular weight marker; lane 2, 35S-methionine-labeled Cx31 protein (the image of lanes 1 and 2 was taken by autoradiography); lane 3, unlabeled Cx31 protein translated using the TNT system; lane 4, HeLa cell lysate transfected with pcDNA3/Cx31; lane 5, the lysate of untransfected Hela cells. Lanes 3, 4, and 5 were transferred to nitrocellulose membrane after electrophoresis and blotted with the Cx31 antibody. Journal of Investigative Dermatology 2001 117, 958-964DOI: (10.1046/j.0022-202x.2001.01468.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Connexin 31 portein in the human epidermis. The expression pattern of Cx31 protein in (a) normal human interfollicular epidermis (scale bar: 20 µm), (b) normal palm epidermis (scale bar: 20 µm), (c) the outer root sheath of the hair follicle (scale bar: 20 µm) (taken from the boxed area in (e), scale bar: 10 µm), and (d) sebaceous gland (scale bar: 20 µm) (taken from the boxed area in (f), scale bar: 10 µm). Journal of Investigative Dermatology 2001 117, 958-964DOI: (10.1046/j.0022-202x.2001.01468.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 The expression pattern of connexin proteins in normal human interfollicular epidermis. Immunofluorescence staining using connexin antibodies: (a) Cx26; (b) Cx30; (c) Cx32; (d) Cx40; (e) Cx43; (f) Cx45. Scale bar: 20 µm. Journal of Investigative Dermatology 2001 117, 958-964DOI: (10.1046/j.0022-202x.2001.01468.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 The expression pattern of connexin proteins in normal palm epidermis. Immunofluorescence staining using connexin antibodies: (a) Cx26; (b) Cx30; (c) Cx32; (d) Cx40; (e) Cx43; (f) Cx45. Scale bar: 20 µm. Journal of Investigative Dermatology 2001 117, 958-964DOI: (10.1046/j.0022-202x.2001.01468.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Connexin mRNA expressed in human interfollicular and palm skin. mRNA transcripts were amplified from human skin (upper image) and palm (low image) by RT-PCR. (–) indicates that the amplifications in the absence of reverse transcriptase were used as controls. Journal of Investigative Dermatology 2001 117, 958-964DOI: (10.1046/j.0022-202x.2001.01468.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Distribution of Cx31 mRNA in palm. The Cx31 mRNA was detected by in situ hybridization: (a) sense; (b) antisense. Scale bar: 10 µm. Journal of Investigative Dermatology 2001 117, 958-964DOI: (10.1046/j.0022-202x.2001.01468.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions