Quantitative grading of a human blastocyst: optimal inner cell mass size and shape Kevin S Richter, Ph.D., Dee C Harris, M.T., Said T Daneshmand, M.D.,

Slides:

Advertisements

Similar presentations

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Advertisements

Human blastocyst morphological quality is significantly improved in embryos classified as fast on day 3 (≥10 cells), bringing into question current embryological.

Diminished ovarian reserve as measured by means of baseline follicle-stimulating hormone and antral follicle count is not associated with pregnancy loss.

Are good patient and embryo characteristics protective against the negative effect of elevated progesterone level on the day of oocyte maturation? Micah.

Study of nucleation status in the second cell cycle of human embryo and its impact on implantation rate Jesus Aguilar, Ph.D., Irene Rubio, Ph.D., Elkin.

A giant oocyte in a cohort of retrieved oocytes: does it have any effect on the in vitro fertilization cycle outcome? Ronit Machtinger, M.D., Joseph.

Nicole Banks, M. D. , George Patounakis, M. D. , Ph. D

MicroRNAs in spent blastocyst culture medium are derived from trophectoderm cells and can be explored for human embryo reproductive competence assessment

Ratio between inner cell mass diameter and blastocyst diameter is correlated with successful pregnancy outcomes of single blastocyst transfers Miriam.

Single blastocyst transfer: a prospective randomized trial

Bruce S. Shapiro, Said T. Daneshmand, Humberto Restrepo, Forest C

Patient and cycle characteristics predicting high pregnancy rates with single-embryo transfer: an analysis of the Society for Assisted Reproductive Technology.

Contrasting patterns in in vitro fertilization pregnancy rates among fresh autologous, fresh oocyte donor, and cryopreserved cycles with the use of day.

Trophectoderm grade predicts outcomes of single-blastocyst transfers

Factors affecting the success of human blastocyst development and pregnancy following in vitro fertilization and embryo transfer 1 Gayle M Jones, Ph.D.,

Reducing multiples: a mathematical formula that accurately predicts rates of singletons, twins, and higher-order multiples in women undergoing in vitro.

Factors associated with birth outcomes from cryopreserved blastocysts: experience from 4,597 autologous transfers of 7,597 cryopreserved blastocysts

Reproductive BioMedicine Online

Dramatic declines in implantation and pregnancy rates in patients who undergo repeated cycles of in vitro fertilization with blastocyst transfer after.

Evidence of impaired endometrial receptivity after ovarian stimulation for in vitro fertilization: a prospective randomized trial comparing fresh and.

Clinical experience and perinatal outcome of blastocyst transfer after coculture of human embryos with human endometrial epithelial cells: a 5-year follow-up.

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Live birth of twins derived from zona-free oocytes

Orhan Bukulmez, M. D. , Qin Li, M. S. , Bruce R. Carr, M. D

Lindsay Kroener, M. D. , Gayane Ambartsumyan, M. D. , Ph. D

Iris G. Insogna, M. D. , Malinda S. Lee, M. D. , M. B. A. , Rebecca M

Predictive value of embryo grading for embryos with known outcomes

Optimizing the number of blastocyst stage embryos to transfer on day 5 or 6 in women 38 years of age and older: a Society for Assisted Reproductive Technology.

Diminished ovarian reserve as measured by means of baseline follicle-stimulating hormone and antral follicle count is not associated with pregnancy loss.

The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility.

Improvements achieved in an oocyte donation program over a 10-year period: sequential increase in implantation and pregnancy rates and decrease in high-order.

Moving to blastocyst biopsy for preimplantation genetic diagnosis and single embryo transfer at sydney IVF Kylie A de Boer, Ph.D., James W Catt, Ph.D.,

Embryo incubation and selection in a time-lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort.

Martin Ivec, B.Sc., Borut Kovacic, Ph.D., Veljko Vlaisavljevic, Ph.D.

Influences on endometrial development during intrauterine insemination: clinical experience of 2,929 patients with unexplained infertility Erin Foran.

Embryo transfer practices and multiple births resulting from assisted reproductive technology: an opportunity for prevention Dmitry M. Kissin, M.D.,

Automatic time-lapse instrument is superior to single-point morphology observation for selecting viable embryos: retrospective study in oocyte donation

Role of coculture in human in vitro fertilization: a meta-analysis

I. Dimitriadis, G. Y. Christou, K. A. Dickinson, M. Thatcher, C. L

Initial IVF-ET experience with assisted hatching performed 3 days after retrieval followed by day 5 embryo transfer Margaret C. Graham, M.S., Kathleen.

Human chorionic gonadotropin levels after blastocyst transfer are highly predictive of pregnancy outcome1 Theocharis C Papageorgiou, M.D., Mark P Leondires,

Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded.

Late stages of embryo progression are a much better predictor of clinical pregnancy than early cleavage in intracytoplasmic sperm injection and in vitro.

Does a frozen embryo transfer ameliorate the effect of elevated progesterone seen in fresh transfer cycles? Mae Wu Healy, D.O., George Patounakis, M.D.,

Should embryos developing to blastocysts on day 7 be cryopreserved and transferred: an analysis of pregnancy and implantation rates George Kovalevsky,

Nondominant small follicles are a promising source of mature oocytes in modified natural cycle in vitro fertilization and embryo transfer Shokichi Teramoto,

Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization.

Samer Alfarawati, M. S. , Elpida Fragouli, Ph. D. , Pere Colls, Ph. D

Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic.

Birth weight is associated with inner cell mass grade of blastocysts

Aaron K. Styer, M. D. , Diane L. Wright, Ph. D. , H. C. L. D. , Anne M

Live babies born per oocyte retrieved in a subpopulation of oocyte donors with repetitive reproductive success J. Ryan Martin, M.D., Jason G. Bromer,

Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology.

Body mass index and uterine receptivity in the oocyte donation model

Dizygotic twin delivery following in vitro fertilization and transfer of thawed blastocysts cryopreserved at day 6 and 7 E.Scott Sills, M.D., Cindy L.

Bruce S Shapiro, M. D. , Kevin S Richter, Ph. D. , Dee C Harris, M. T

Obesity does not impact implantation rates or pregnancy outcome in women attempting conception through oocyte donation Allison Styne-Gross, M.D., Karen.

Qiong Zhang, M.D., Xiaoyue M. Guo, B.A., Yanping Li, M.D.

Vasiliki A. Moragianni, M. D. , M. S. , Stephanie-Marie L. Jones, M. D

Is tubal embryo transfer of any value

Barry Behr, Ph.D., H.C.L.D., Amin A Milki, M.D.

Martha Luna, M. D. , Marlena Duke, M. Sc. , Alan Copperman, M. D

Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery.

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Day 3 morphology is a poor predictor of blastocyst quality in extended culture James Graham, M.S., Taer Han, B.S., Richard Porter, Michael Levy, M.D.,

William B Schoolcraft, M.D., David K Gardner Fertility and Sterility

Peter M. Martin, M.D., H.Gilbert Welch, M.D. Fertility and Sterility

Conversion from assisted reproductive technology to intrauterine insemination in low responders: Is it advantageous? John M. Norian, M.D., Eric D. Levens,

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Male age negatively impacts embryo development and reproductive outcome in donor oocyte assisted reproductive technology cycles John L. Frattarelli,

Presentation transcript:

Quantitative grading of a human blastocyst: optimal inner cell mass size and shape Kevin S Richter, Ph.D., Dee C Harris, M.T., Said T Daneshmand, M.D., Bruce S Shapiro, M.D. Fertility and Sterility Volume 76, Issue 6, Pages 1157-1167 (December 2001) DOI: 10.1016/S0015-0282(01)02870-9

FIGURE 1 Frequency histograms of ICM dimensions among blastocysts transferred on day 5: size (A), calculated as the product of longest length and widest perpendicular width, in intervals of 1,000 μm2; and shape (B), according to the RI, calculated as length divided by width, in intervals of 0.1. Dark shading indicates embryos derived from patients’ oocytes; light shading indicates embryos derived from donated oocytes. Richter. Optimal inner cell mass size and shape. Fertil Steril 2001. Fertility and Sterility 2001 76, 1157-1167DOI: (10.1016/S0015-0282(01)02870-9)

FIGURE 2 Implantation rates among known-outcome blastocysts transferred on day 5, grouped into 500-μm2 ICM size intervals (or 1,000-μm2 intervals <2,000 μm2 and >7,000 μm2) plotted against the mean ICM size of the embryos in each group. Point size is proportional to the number of embryos represented. The trend line was determined by logistic regression analysis (P=.01). Richter. Optimal inner cell mass size and shape. Fertil Steril 2001. Fertility and Sterility 2001 76, 1157-1167DOI: (10.1016/S0015-0282(01)02870-9)

FIGURE 3 Implantation rates among known-outcome blastocysts transferred on day 5, grouped by ICM size (A), ICM shape (B), or both ICM size and shape (C). (A), ICM size: ≤4,500 μm2 (n = 70) or >4,500 μm2 (n = 69). P=.006 for difference. (B), ICM shape: RI of <1.04 (n = 15), RI from 1.04 to 1.20 (n = 57), or RI of >1.20 (n = 67). Implantation rates were significantly higher for slightly oval ICMs compared with either rounder ICMs (P=.012) or more elongated ICMs (P=.006). (C), ICM size and shape: optimal size and shape (OptS&S, n = 35), optimal size only (OptSize, n = 34), optimal shape only (OptShape, n = 22), or neither optimal size nor shape (SubOpt, n = 48). Implantation rates were significantly higher for OptS&S embryos compared with OptSize embryos (P=.011), OptShape embryos (P=.038), or SubOpt embryos (P=.0001). Richter. Optimal inner cell mass size and shape. Fertil Steril 2001. Fertility and Sterility 2001 76, 1157-1167DOI: (10.1016/S0015-0282(01)02870-9)

FIGURE 4 Estimated implantation rates of embryos transferred on day 5 (based on multiple linear regression analysis, with 95% confidence intervals) according to ICM size and/or shape categories. (A), size groupings only: ≤4,500 μm2 (n = 136) or >4,500 μm2 (n = 116); (B), shape groupings only: RI of <1.04 (n = 21), RI from 1.04 to 1.20 (n = 117), or RI of >1.20 (n = 114); or (C), combined size and shape groupings: OptS&S (n = 62), OptSize (n = 54), OptShape (n = 55), or SubOpt (n = 81). Richter. Optimal inner cell mass size and shape. Fertil Steril 2001. Fertility and Sterility 2001 76, 1157-1167DOI: (10.1016/S0015-0282(01)02870-9)

FIGURE 5 Graphic representation of the length-to-width proportions associated with representative values of RI. Dark shading indicates optimal shape, and light shading, suboptimal shape. Richter. Optimal inner cell mass size and shape. Fertil Steril 2001. Fertility and Sterility 2001 76, 1157-1167DOI: (10.1016/S0015-0282(01)02870-9)