Maria Teresa Zenzes, Ph. D. , Ryszard Bielecki, Robert F Casper, M. D

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Effects of chilling to 0°C on the morphology of meiotic spindles in human metaphase II oocytes Maria Teresa Zenzes, Ph.D., Ryszard Bielecki, Robert F Casper, M.D., S.P Leibo, Ph.D. Fertility and Sterility Volume 75, Issue 4, Pages 769-777 (April 2001) DOI: 10.1016/S0015-0282(00)01800-8

Figure 1 Fluorescent micrographs of control, untreated human oocytes to show examples of spindle abnormalities. Chromosomes are stained in blue and spindles in green. Arrows in panels A and B indicate chromosomes or single chromatids displaced out of the spindle. In panel B, both the spindle and the first polar body of an oocyte are shown. In panels C and E, the spindles appear to have a tetrapolar or tripolar arrangement, respectively. The oocyte in panel D seems to have an unusually large number of chromosomes. Panel F shows a normal, bipolar spindle with the chromosomes aligned on the equatorial plane. (Bar = 5 μm.) Zenzes. Chilling of meiotic spindles in oocytes. Fertil Steril 2001. Fertility and Sterility 2001 75, 769-777DOI: (10.1016/S0015-0282(00)01800-8)

Figure 2 Fluorescent micrographs of meiotic spindles of oocytes that had been cooled rapidly to 0°C and held for the indicated times (1 min, 2 min, etc.). The panels labeled control 1 and control 2 are of two untreated oocytes. The micrographs were made with an optical sectioning microscope, together with deconvolution software. Chromosomes are stained in red and spindles in green. Arrows indicate precocious segregation of single chromatids. (Bar = 5 μm.) Zenzes. Chilling of meiotic spindles in oocytes. Fertil Steril 2001. Fertility and Sterility 2001 75, 769-777DOI: (10.1016/S0015-0282(00)01800-8)