Phenotype of rTT.C-specific plasma blasts.

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Phenotype of rTT.C-specific plasma blasts. Phenotype of rTT.C-specific plasma blasts. Differential expression of various Ags on rTT.C-specific PBMCs from a representative donor 6 days after TT booster immunization. Formaldehyde-fixed PBMCs were gated (A) and stained with anti-CD27 Cy5 mAb, biotinylated anti-CD19 mAb/SA PerCP, rTT.C-Dig, and anti-Dig PE in the presence of saponin. rTT.C-specific CD19+/CD27high or CD19+/CD27high PBMCs with undefined specificity were identified by gating as indicated in panels B and C. Forward scatter, indicating the cell size, expression of HLA-DR, CD20, CD38, CD95, or intracellular IgG is shown for rTT.C-specific CD19+/CD27high (▦) or CD19+/CD27high PBMCs with undefined specificity (▪) (D-I). MFIs are indicated by the numbers in the top corners. Marcus Odendahl et al. Blood 2005;105:1614-1621 ©2005 by American Society of Hematology