Overexpression of microRNA-21 protects spinal cords against transient ischemia Fang He, MD, PhD, Yixing Ren, MD, Enyi Shi, MD, PhD, Kun Liu, MD, Lihui.

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Presentation transcript:

Overexpression of microRNA-21 protects spinal cords against transient ischemia Fang He, MD, PhD, Yixing Ren, MD, Enyi Shi, MD, PhD, Kun Liu, MD, Lihui Yan, MD, Xiaojing Jiang, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 152, Issue 6, Pages 1602-1608 (December 2016) DOI: 10.1016/j.jtcvs.2016.07.065 Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Effects of overexpression of microRNA-21 on hind-limb motor function assessed with the Motor Deficit Index before spinal cord ischemia and 6, 12, 24, and 48 hours after reperfusion. A 14-minute aortic occlusion resulted in severe hind extremity neurologic deficits in both the control and control vector groups (P = .000 vs the sham group at the 4 time points after reperfusion). Intrathecal injection of lentivirus of pre-microRNA-21 (pre-miR-21) remarkably decreased the Motor Deficit Index scores at the 4 observation time points after reperfusion (P = .000 vs control group). The Journal of Thoracic and Cardiovascular Surgery 2016 152, 1602-1608DOI: (10.1016/j.jtcvs.2016.07.065) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Expression of microRNA-21 in spinal cords. Intrathecal injection of lentivirus of before administration of microRNA-21 (pre-miR-21) markedly increased the expression of microRNA-21 in the pre-miR-21 group (P = .000 vs the control group). The Journal of Thoracic and Cardiovascular Surgery 2016 152, 1602-1608DOI: (10.1016/j.jtcvs.2016.07.065) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Expressions of caspase-3, Fas ligand (Faslg), and programmed cell death 4 (PDCD4) in spinal cords. A, Representative western blot picture showing caspase-3, Faslg, and PDCD4 expressions. B, Densitometric quantification of caspase-3 expression. C, Densitometric quantification of Faslg expression. D, Densitometric quantification of PDCD4 expression. The expressions of caspase-3, Faslg, and PDCD4 in spinal cords increased dramatically after the transient ischemia in the control and control vector groups (P = .000 vs sham group), whereas pretreatment with lentivirus of pre-microRNA-21 (pre-miR-21) significantly reduced the expressions of caspase-3, Faslg, and PDCD4 in the pre-miR-21 group (P = .000 vs the control group). GADPH, Glyceraldehyde 3-phosphate dehydrogenase. The Journal of Thoracic and Cardiovascular Surgery 2016 152, 1602-1608DOI: (10.1016/j.jtcvs.2016.07.065) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 The number of large motor neurons in the ventral gray matter of lumber spinal cords. Compared with the control group, the number of large motor neurons was much higher in the pre-microRNA-21 (pre-miR-21) group (P = .000). The Journal of Thoracic and Cardiovascular Surgery 2016 152, 1602-1608DOI: (10.1016/j.jtcvs.2016.07.065) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Overexpression of microRNA-21 protects motor function after spinal cord ischemia. *P < .01 compared with the control group. The Journal of Thoracic and Cardiovascular Surgery 2016 152, 1602-1608DOI: (10.1016/j.jtcvs.2016.07.065) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions