Identification of 2 new sesame seed allergens: Ses i 6 and Ses i 7

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Presentation transcript:

Identification of 2 new sesame seed allergens: Ses i 6 and Ses i 7 Kirsten Beyer, MD, Galina Grishina, MS, Ludmilla Bardina, MS, Hugh A. Sampson, MD Journal of Allergy and Clinical Immunology Volume 119, Issue 6, Pages 1554-1556 (June 2007) DOI: 10.1016/j.jaci.2007.03.041 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Shown is the alignment for the newly identified sesame seed allergens Ses i 6 and Ses i 7 with know allergens of peanut (Ara h 3), walnut (Jug r 4), and hazelnut (Cor a 9) by using Vector NTI Advance. All aligned allergens belong to the 11 S globulins. Journal of Allergy and Clinical Immunology 2007 119, 1554-1556DOI: (10.1016/j.jaci.2007.03.041) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 The recombinant proteins Ses i 6 and Ses i 7 were separated on SDS-PAGE and transferred to Immobilon-P membranes. Lane 1 shows the molecular weight (MW) markers, lane 2 shows the Coomassie Blue stain for total protein analysis, lanes 3 to 27 show immunolabeling with sera from 24 patients with sesame seed allergy, and lanes 28 and 29 show the atopic and nonatopic controls, respectively. Journal of Allergy and Clinical Immunology 2007 119, 1554-1556DOI: (10.1016/j.jaci.2007.03.041) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions