MetaPhase clustering results on the M-Y draft metagenome assembly.

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MetaPhase clustering results on the M-Y draft metagenome assembly. MetaPhase clustering results on the M-Y draft metagenome assembly. (A) Using Hi-C links to cluster contigs into 12 clusters, one for every species with a substantial presence in the draft assembly. Each contig is shown as a dot, with size indicating contig length, colored by species. Edge widths represent the densities of Hi-C links between the contigs shown. Only 2400 contigs are shown: the 200 largest contigs that map uniquely to each species. (B) Validation. This heatmap indicates what fraction of the sequence in each MetaPhase cluster maps uniquely to each of the reference genomes of the 12 present yeast species. Note that not all sequence is expected to map uniquely to one species. x-axis: the 12 yeast species. y-axis: the MetaPhase clusters. (C and D) Lachesis (Burton et al. 2013) reconstruction of individual species’ genomes within the M-Y metagenome assembly. These heatmaps show the Hi-C link density among the contigs in the MetaPhase clusters corresponding to S. stipitis (C) and K. wickerhamii (D). The x-axis and y-axis show the clustering and ordering of contigs by Lachesis. Dotted black lines demarcate chromosomal clusters. Note the expected signals of enrichment within each chromosome and on the main diagonal. The assembly in (C) is similar to the S. stipitis reference genome (Figure S7), whereas the assembly in (D) has far higher chromosome-scale contiguity than the best available K. wickerhamii reference (Baker et al. 2011). Joshua N. Burton et al. G3 2014;4:1339-1346 ©2014 by Genetics Society of America