WD confers vulnerability to OFT anxiety-like behaviors following traumatic stress exposure. WD confers vulnerability to OFT anxiety-like behaviors following traumatic stress exposure. A, PS exposure reduced the number of entries to the center of the OFT (PS: F(1,23) = 14.48, p = 0.0009; diet: F(1,23) = 0.11, p = 0.75; diet × PS interactions: F(1,23) = 2.04, p = 0.17). Post hoc comparisons indicated that the effect of PS was particularly robust in WDE rats when compared with WDU controls (Tukey’s test adjusted, p = 0.0036). B, ANOVA revealed that PS decreased the duration in the center (F(1,23) = 5.02, p = 0.035), while diet (F(1,23) = 0.83, p = 0.37) and diet–PS exposure interactions (F(1,23) = 0.97, p = 0.34) showed no significant main effects. C, PS exposure reduced the total distance traveled in the open field arena (F(1,23) = 32.85, p = 0.0001). Analyses revealed no significant main effect of the diet (F(1,23) = 0.37, p = 0.55) or diet–PS exposure interactions (F(1,23) = 0.049, p = 0.83) on locomotor activity in the open field arena. D, PS exposure increased indices of anxiety on the OFT (PS: F(1,23) = 25.97, p < 0.0001). The main effects of the diet (F(1,23) = 0.37, p = 0.55) and diet–PS exposure interactions (F(1,23) = 1.35, p = 0.26) showed no statistical significance on the OFT anxiety index. E, OFT heat map analyses show that thigmotaxis was particularly robust in the WDE group. All data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, n = 6–10 rats/group. Priya Kalyan-Masih et al. eneuro 2016;3:ENEURO.0125-16.2016 ©2016 by Society for Neuroscience