NOD2 deletion exacerbates diet‐induced bacterial translocation, inflammation, and impairs insulin action in the liver A–CQuantification of bacterial DNA.

Slides:



Advertisements
Similar presentations
Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.
Advertisements

Reduction in immune cell infiltration after stab wound injury in CCR2−/− mice increases proliferation at the injury site Reduction in immune cell infiltration.
Morphology of white adipose tissue and muscle in S1/3 KO mice of different ages Morphology of white adipose tissue and muscle in S1/3 KO mice of different.
Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.
Reduced scar formation in the injured GM of CCR2−/− mice
Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice.
PERK activator reduces behavioral deficits and neurodegeneration in vivo PERK activator reduces behavioral deficits and neurodegeneration in vivo A–CYoung.
Neuro‐inflammatory cytokines, brain microglial activation, and working memory deficits are normalized using LV.IDS.ApoEII, but not LV.IDS in MPS II mice.
The gene encoding TP53INP1 is expressed in pancreatic endocrine cells A, B(A, B) Immunocytofluorescent staining of TP53INP1 (red) and insulin (green) in.
PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection Proportion.
[68Ga]Pentixafor PET imaging of MM xenografts Real‐time PCR analysis of cxcr4 transcript expression levels in HeLa (negative control) and in MM cell lines.
TFE3 regulates glucose homeostasis
Expression levels of the transgenic DIA1(R1204X) mutant in the inner ear of TG mice Expression levels of the transgenic DIA1(R1204X) mutant in the inner.
AAV8‐hAAT‐FGF21 treatment improves liver fibrosis
Metabolic profile of Tfe3 KO mice fed a HFD
SPRY2 deficiency mediates androgen autonomous CRPC
Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis.
Volume 20, Issue 4, Pages (October 2014)
Metabolic profile of Tfe3 KO mice
Tumour‐induced IL6 increases systemic cholesterol levels
Effects of GM1 on astroglial and microglial markers
TFE3 prevents diet‐induced obesity and metabolic syndrome
Volume 41, Issue 6, Pages (December 2014)
Impaired glucose tolerance in adult S1/3 KO mice on a normal chow
SPRY2 deficiency mediates CRPC
Volume 25, Issue 5, Pages e3 (May 2017)
Stromal fibromuscular AR regulates epithelium proliferation, ECM remodelling, neovasculature formation and immune cell infiltration in Pten deficient mice.
TR specifically inhibits NLRP3 inflammasome activation
SPRY2 deficiency‐induced IL6 drives CRPC by elevating tumoral HSD3B1 and cholesterol levels SPRY2 deficiency‐induced IL6 drives CRPC by elevating tumoral.
WT‐HSCT, LV. IDS, and LV. IDS
Volume 18, Issue 10, Pages (March 2017)
Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis.
Volume 14, Issue 10, Pages (March 2016)
Intra‐eWAT administration of FGF21 vectors in ob/ob mice
Hdac3 deletion decreases AKT phosphorylation and tumor growth in Pten knockout prostate cancer Hdac3 deletion decreases AKT phosphorylation and tumor growth.
Rescuing mGPDH deficiency improves skeletal muscle regeneration during obesity and diabetes Rescuing mGPDH deficiency improves skeletal muscle regeneration.
The psoriatic phenotype of DKO
Impaired ECM protein synthesis in infarcted area of S100a4cre+/− × Fstl1flox/flox mice Impaired ECM protein synthesis in infarcted area of S100a4cre+/−
Treatment with AAV8‐hAAT‐FGF21 improves insulin sensitivity and glucose tolerance Treatment with AAV8‐hAAT‐FGF21 improves insulin sensitivity and glucose.
Lipogenic genes and SREBP1 protein are upregulated in HF‐R
The preventive role of TR in HFD‐treated mice
IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.
mGPDH is not essential to muscle development
Volume 22, Issue 2, Pages (August 2015)
High‐fat diet augments gut permeability, metabolic endotoxemia, and systemic inflammation High‐fat diet augments gut permeability, metabolic endotoxemia,
Volume 14, Issue 4, Pages (October 2011)
GPR120 deficiency alters the expression of genes involved in glucose and lipid metabolism in BAT GPR120 deficiency alters the expression of genes involved.
Volume 16, Issue 3, Pages (July 2016)
Quantification of phenotypes by genes and developmental stage
MiR‐34a‐5p functionally contributes to arrested lung alveolarization in response to hyperoxia miR‐34a‐5p functionally contributes to arrested lung alveolarization.
Therapeutic role of TR in HFD‐established diabetic mice
Rescue of motor neurons from death The ventral root of the fifth lumbar segment (L5). Rescue of motor neurons from death The ventral root of the fifth.
Tissue‐specific differences in the levels of CoQ9, CoQ10, and DMQ9, and in the DMQ9/CoQ9 ratio after β‐RA treatment in Coq9R239X mice Tissue‐specific differences.
Volume 41, Issue 6, Pages (December 2014)
Volume 22, Issue 2, Pages (August 2015)
Systemic GADD45β deletion affects metabolic regulation under conditions of heightened lipid metabolism Systemic GADD45β deletion affects metabolic regulation.
SPRY2 deficiency mediates CRPC
KlbHET mice exhibit altered estrous cycle and subfertility
SEMA3C regulates prostate cancer cell growth
Niacin suppresses pro‐inflammatory cytokine expression in macrophages in DSS‐induced colitis in mice Niacin suppresses pro‐inflammatory cytokine expression.
HFD feeding induced insulin resistance in TRIB3 MOE mice.
Hepatic fuel metabolism in male 5αR1-KO and WT mice
Mice fed GP-SPI diet show improved fasting glucose and oral glucose tolerance. Mice fed GP-SPI diet show improved fasting glucose and oral glucose tolerance.
Insulin resistance and hepatic steatosis in ASKO mice.
Loss of Phb2 in β-cells induces development of diabetes over a 3-week period in β-Phb2−/− mice. Loss of Phb2 in β-cells induces development of diabetes.
Defective NOD2 peptidoglycan sensing promotes diet‐induced inflammation, dysbiosis, and insulin resistance Unweighted UniFrac principal coordinates analysis.
Defective NOD2 peptidoglycan sensing promotes diet‐induced inflammation, dysbiosis, and insulin resistance ATranscript levels of NOD2 in various tissues.
Defective NOD2 peptidoglycan sensing promotes diet‐induced inflammation, dysbiosis, and insulin resistance A–DThe number of ampicillin‐resistant E. coli.
Defective NOD2 peptidoglycan sensing promotes diet‐induced inflammation, dysbiosis, and insulin resistance APrincipal coordinates analysis (PCoA) performed.
Fig. 5 Effects of in vivo blockade of TLR9 on adipose tissue inflammation and insulin resistance in WT mice. Effects of in vivo blockade of TLR9 on adipose.
Presentation transcript:

NOD2 deletion exacerbates diet‐induced bacterial translocation, inflammation, and impairs insulin action in the liver A–CQuantification of bacterial DNA in the gonadal white adipose tissue (A) (WAT, n = 14–16 mice for all groups), mesenteric adipose tissue (B) (MAT, n = 10–15 mice for all groups), and liver (C) (n = 10–12 mice for all groups) of chow‐fed and 16 week HFD‐fed WT and NOD2−/− mice, *P = 0.0002. NOD2 deletion exacerbates diet‐induced bacterial translocation, inflammation, and impairs insulin action in the liver A–CQuantification of bacterial DNA in the gonadal white adipose tissue (A) (WAT, n = 14–16 mice for all groups), mesenteric adipose tissue (B) (MAT, n = 10–15 mice for all groups), and liver (C) (n = 10–12 mice for all groups) of chow‐fed and 16 week HFD‐fed WT and NOD2−/− mice, *P = 0.0002. DQuantification of NOD2 transcripts in hepatocyte and non‐hepatocyte cell populations of liver from chow‐fed (n = 10) and HFD‐fed (n = 6) WT mice, *P = 0.006. EQuantification of macrophage and inflammatory markers in liver of chow‐fed (n = 5 in both genotypes) or HFD‐fed (n = 11 in both genotypes) WT and NOD2−/− mice (n = 6 for IL‐6 analysis), *P = 0.02, **P = 0.0001, #P = 0.02, and ##P = 0.002. FRepresentative liver IHC for the macrophage marker F4/80 (representative of n = 5 WT and n = 6 NOD2−/− mice). Scale bar equals 50 μm. GQuantification of hepatic triglycerides (TAG) in HFD‐fed WT (n = 6) and NOD2−/− (n = 6) mice, *P = 0.0002. HQuantification of hepatic G6P transcript levels in chow‐fed (n = 5) and HFD‐fed (n = 11) WT and NOD2−/− mice, *P = 0.02. I, JQuantification (I) and immunoblots (J) of insulin‐stimulated pFOXO1Ser256 in liver lysates after vena cava injection of insulin (0.5 IU/kg) in HFD‐fed WT (n = 4) and NOD2−/− (n = 4) mice, *P = 0.0498. KBlood glucose and quantification of the AUC during a 120‐min pyruvate tolerance test (PTT, 2.0 g/kg pyruvate, i.p.) in HFD‐fed WT (n = 6) and NOD2−/− mice (n = 6), *P = 0.0005. Data information: An unpaired t‐test was used for comparisons with two conditions, whereas a 1‐way ANOVA was used for comparisons with more than two conditions. Tukey's post‐hoc test was used. *Significantly different from HFD‐fed WT mice; In (D), *significantly different from chow‐fed, WT mice. #Significantly different from chow‐fed WT mice. Values are mean ± SEM. Emmanuel Denou et al. EMBO Mol Med. 2015;emmm.201404169 © as stated in the article, figure or figure legend