Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection Kenneth L. Muldrew, Jean F. Simpson, Charles.

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Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection Kenneth L. Muldrew, Jean F. Simpson, Charles W. Stratton, Yi-Wei Tang The Journal of Molecular Diagnostics Volume 7, Issue 5, Pages 641-645 (November 2005) DOI: 10.1016/S1525-1578(10)60599-5 Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Photomicrographs of lesional tissue isolated from the popliteal fossa at autopsy. A: Hemorrhage and necrosis (H&E). B: The overall sparsely cellular inflammatory infiltrate with necrosis in the fascia and associated adipose tissue (H&E). C: Portion of adipose tissue with the predominantly mononuclear inflammatory infiltrate, associated vasculitis, and a thrombosed vessel (H&E). D: Gram-positive cocci in clusters (Phenol Green gram stain). Original magnifications: ×20 (A); ×40 (B); ×200 (C); ×400 (D). The Journal of Molecular Diagnostics 2005 7, 641-645DOI: (10.1016/S1525-1578(10)60599-5) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Streptococcal pyrogenic exotoxin gene detection by PCR. Three spe genes and a human β-actin gene were detected by individual PCR, respectively. Lanes 2 to 5 contained tissue DNA specimens amplified by speA-, speB-, speC-, and β-actin-specific primer sets. Lanes 6 and 7 contained a DNA specimen extracted from ATCC123536 amplified by speA- and speB-specific primer sets. Lane 8 contained a DNA specimen extracted from BAA-5727 amplified by a speC-specific primer set. Molecular sizes (lane 1) are in bp. The Journal of Molecular Diagnostics 2005 7, 641-645DOI: (10.1016/S1525-1578(10)60599-5) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions