Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical.

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Presentation transcript:

Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial Richard T. Scott, M.D., Kathleen M. Upham, B.S., Eric J. Forman, M.D., Tian Zhao, M.S., Nathan R. Treff, Ph.D. Fertility and Sterility Volume 100, Issue 3, Pages 624-630 (September 2013) DOI: 10.1016/j.fertnstert.2013.04.039 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Study design to assess the impact of biopsy at the cleavage stage (day 3) on embryonic reproductive potential. The randomized paired experimental design allowed each patient to serve as her own control. An equivalent methodology was used to determine if trophectoderm biopsy at the blastocyst stage affected embryonic potential. ICSI = intracytoplasmic sperm injection. Fertility and Sterility 2013 100, 624-630DOI: (10.1016/j.fertnstert.2013.04.039) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Consolidated Standards of Reporting Trials flow diagram detailing patient recruitment and progression through the study protocol. Of 116 enrolled patients, 113 completed the study and provided evaluable data. Fertility and Sterility 2013 100, 624-630DOI: (10.1016/j.fertnstert.2013.04.039) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Implantation rates following a randomized paired analysis of the effects of cleavage- and blastocyst-stage biopsies on embryo reproductive potential. Sustained implantation and delivery of the biopsied embryo were significantly reduced compared with its control sibling when biopsy was performed on day 3 at the cleavage stage (McNemar chi-square: P<.03). A similar paired analysis demonstrated that the developmental potential of embryos undergoing trophectoderm biopsy at the blastocyst stage was equivalent to the nonbiopsied control siblings. Fertility and Sterility 2013 100, 624-630DOI: (10.1016/j.fertnstert.2013.04.039) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions