Suggested diagnostic protocol for investigation of suspected SLE

Slides:

Advertisements

Similar presentations

ANA Testing Carrie Marshall 1/18/08.

Advertisements

Value of inflammatory markers Useful for diagnosis of inflammatory vs non inflammatory conditions Remember NON-SPECIFIC, increased in infection, inflammation,

Performance Characteristics of the FARRZYME™ High Avidity Anti-dsDNA IgG Antibody ELISA Brenda B. Suh-Lailam 1, Tyson R. Chiaro 1, K. Wayne Davis 1, Andrew.

Autoantibodies in LE R3 Anchisa Srivipatana. Antinuclear antibody (ANA)

ELISA Enzyme-linked Immunosorbant Assay Detects the presence of minutes quantities of either an antibody or an antigen Important diagnostic tool in many.

 YEOH HUI SHIH –INTRODUCTION TO HIV  RAJAMANI- ELISA  TIEN WEI PING – WESTERN BLOTTING  YEO HUI YUN –OVERVIEW & CONCLUSION.

P437 Complement deficiency in early-onset lupus: experience from a tertiary care centre in North India 1Sagar Bhattad, 1Amit Rawat, 1Anju Gupta, 1Deepti.

Galactomannan Antigen Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis after Hematopoietic Stem Cell Transplantation Patrick.

Copyright © 2012 American Medical Association. All rights reserved.

3. AUTOIMMUNITY Immune Mechanisms Tolerance 4. Rheumatologic diseases

Table 1 Specificity of IgM immunoblot assay of sera collected during the winter and early spring months from volunteers without clinical evidence of Lyme.

Copyright © 2004 American Medical Association. All rights reserved.

IMMUNE DISORDERS CHAPTER 18

Allergy to Surgical Implants

Characteristics of advanced fibrosis in usual interstitial pneumonia of idiopathic pulmonary fibrosis include a subpleural distribution of fibrosis (A,

Stratified mucinous intraepithelial lesion in which stratified epithelium resembling cervical intraepithelial neoplasia contains abundant mucin globules.

Volume 79, Issue 6, Pages (March 2011)

(A) EULAR response based on DAS28 (ESR, otherwise CRP) and (B) Boolean remission, at 6 months in patients treated with abatacept as a first-line biologic.

Demonstration of Helicobacter pylori by the four staining methods: (A) modified Giemsa, (B) anti-H pylori antibody immunostain, (C) modified McMullen's.

(A,B) mRNA in situ hybridisation (ISH) with Epstein–Barr virus-encoded small RNA-1 probe in a case of Hodgkin’s lymphoma focally affecting the bone marrow.

(A) The two tumours at the end of the ascending and transverse colon are indicated by the arrows. (A) The two tumours at the end of the ascending and transverse.

Pilomatricoma: (A) a multilobulated mass in fibrous stroma reaching the subcutis; (B) basaloid and ghost cells; (C) mitoses in an early lesion; and (D)

(A–F) Hairy-cell leukaemia, haematoxylin and eosin (H&E) staining (A) ×40; (B) ×200; (C) TRAP, ×200; (D) CD25, ×200; (E) DBA44, ×200; (F) CD20, ×200.

Antibiotic hypersensitivity in CF: Drug-induced life-threatening hemolytic anemia in a pediatric patient Alma Chavez, Amir Mian, Amy M. Scurlock, Douglas.

The figure shows representative tissue from multiple gastric biopsies in which the expression of parathyroid hormone related peptide (PTHrP) was investigated.

(A) Proposed new ANA result interpretation algorithm that includes the anti-DFS70 results. (A) Proposed new ANA result interpretation algorithm that includes.

(A) Histiocytes of duct ectasia, where in the absence of an intact duct, the histiocytic population can resemble the tumour cells of histiocytoid breast.

Case 1. Case 1. (A) Low power photomicrograph of renal medulla showing a poorly formed granuloma with several disrupted and destroyed renal tubules within.

Frozen sections of (A) fetal intestine, (B) tonsil, and (C) mantle cell lymphoma, stained using anti-CD3 (immunoperoxidase; brown) followed by anti-CD5.

Immunological testing

Total weekly normal working hours in categories 1 (open bars: the department is open for its full repertoire of services with staff working their weekly.

An oncocytic medullary carcinoma is characterised by polygonal discohesive cells with poorly defined cell borders and relatively bland nuclei, which are.

T-cell/histiocyte rich large B-cell lymphoma.

IgG and IgM Autoantibody Differences in Discoid and Systemic Lupus Patients Benjamin F. Chong, Lin-chiang Tseng, Thomas Lee, Rebecca Vasquez, Quan Z.

Journal of the American College of Surgeons

Mismatch repair immunohistochemistry (MMR IHC).

Jack C. O’Brien, Benjamin F. Chong

Changes in evaluation indicators from baseline to 12 weeks per visit.

A clinical overview of autoantibodies in general practice rheumatology

Detail from the duodenal serrated adenoma in fig 1 showing elongated fronds with lateral crenate, sawtooth-like notches as a result of scalloped epithelial.

Silicatosis. Silicatosis. Similar to sarcoidosis in distribution, the nodules of silicate disease can be distinguished by the common occurrence of dark.

Characteristics of advanced fibrosis in rheumatic diseases include marked lymphoid hyperplasia (A, 20×, H&E), peribronchiolar lymphoid hyperplasia with.

High-power view of high-grade dysplasia with intramucosal adenocarcinoma. High-power view of high-grade dysplasia with intramucosal adenocarcinoma. The.

By immunohistochemistry, 50% of inflammatory myofibroblastic tumours show expression of ALK, which correlates with ALK gene rearrangement. By immunohistochemistry,

Galactomannan Antigen Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis after Hematopoietic Stem Cell Transplantation Patrick.

Percentage of medical laboratory assistants to medical laboratory scientific officers for each of the main disciplines within pathology:(A) biochemistry,

Non-medical productivity in microbiology: requests for each non-medical/non-clinical scientist whole time equivalent—that is, for each whole time equivalent.

Jack C. O’Brien, Benjamin F. Chong

(A–D) Atypical paracortical hyperplasia pattern and (E, F) burnt out histiocytic pattern of lymphadenopathy in adult onset Still’s disease (AOSD). (A–D)

Serum levels of (A), C reactive protein (CRP), (B), erythrocyte sedimentation rate (ESR) and (C), calprotectin of cohort 1 (SPACE) with patients with early.

Algorithm for the investigation of patients with elevated cardiac troponin concentrations in the context of an alternative acute illness. Algorithm for.

DAS28-CRP cut-off values corresponding to the DAS28-ESR cut-off values for remission, LDA and HDA, average of three statistical approaches. DAS28-CRP cut-off.

Receiver operating curve of soluble C3, C4, antibodies to double-stranded DNA (anti-dsDNA) compared with complement C4d levels on erythrocytes (EC4d) and.

Analysis of PD-L1 tumour-cell membrane staining concordance between PD-L1 immunohistochemistry 22C3 and 28-8 assays across all matched tumour biopsies.

Pairwise comparison for lung cancer or unspecified cancer diagnosis for most frequent biopsy sites (lung or lymph node). Pairwise comparison for lung cancer.

PAX8 (mAb) immunohistochemistry in POMTs, eMOMCs and ePMCs.

Antinuclear antibody (ANA) patterns (using HEp-2 ANA assay kit) and anti-dsDNA (analysed by Crithidia luciliae assay) in sera of the lpr-water, lpr-CU.

Cox proportional-hazards model of time to first RA flare after treatment withdrawal for patients who entered the re-treatment period (n=146). Cox proportional-hazards.

Sensitivity and specificity plots of human epididymis protein 4 (HE4) (A and B, respectively) and carbohydrate antigen 125 (CA-125) (C and D, respectively)

Multivariate assay panel for systemic lupus erythematosus (SLE) diagnosis. Multivariate assay panel for systemic lupus erythematosus (SLE) diagnosis. Two-tier.

Classification tree with the selected characteristics.

A 38-year-old man, with recurrent inflammatory back pain and alternating buttock pain for 2 years. A 38-year-old man, with recurrent inflammatory back.

Patient disposition over the 52-week study period

Chromatogram from the ion exchange high performance liquid chromatography Bio-Rad Variant II using the high resolution β thalassaemia programme in a patient.

Molecular biology: A brief overview

Percentage of patients achieving remission by conversion to ACPA seronegative status. Percentage of patients achieving remission by conversion to ACPA.

A representative case of coexisting granulomatous inflammation (black arrowheads) and squamous cell carcinoma (white arrows) in a lobectomy specimen in.

Low grade cervical glandular intraepithelial neoplasia in which there is mild nuclear atypia and easily identifiable mitotic figures and apoptotic bodies.

(A and B) Low-grade serrated dysplasia, on the other hand, is characterised by ovoid enlarged nuclei, vesicular dispersed chromatin and occasional mitoses.

Global Autoimmune Disease Diagnostics Market accounted for USD 3300 million and expected to register 7.6% CAGR during the forecast period 2018–2023 PREPARED.

Presentation transcript:

Suggested diagnostic protocol for investigation of suspected SLE Suggested diagnostic protocol for investigation of suspected SLE. *Confirm weak positives or possible false positives by IgG CLIF; **confirm weak positives or possible false positives by ID/CIE/IB. Suggested diagnostic protocol for investigation of suspected SLE. *Confirm weak positives or possible false positives by IgG CLIF; **confirm weak positives or possible false positives by ID/CIE/IB. Antiphospholipid/lupus anticoagulant assays might be necessary if relevant clinical features of an antiphospholipid syndrome are present. ANA, antinuclear antibody; CIE, countercurrent immunoelectrophoresis; CLIF, Crithidia luciliae immunofluorescence; CRP, C reactive protein; dsDNA, double stranded DNA; ELISA, enzyme linked immunosorbent assay; ENA, extractable nuclear antigen; ESR, erythrocyte sedimentation rate; ID, immunodiffusion; IB, immunoblotting; PCNA, proliferating cell nuclear antigen. William Egner J Clin Pathol 2000;53:424-432 Copyright © by the BMJ Publishing Group Ltd & Association of Clinical Pathologists. All rights reserved.