Volume 17, Issue 3, Pages (March 2010)

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Presentation transcript:

Volume 17, Issue 3, Pages 265-273 (March 2010) Two Alternative Starter Modules for the Non-Ribosomal Biosynthesis of Specific Anabaenopeptin Variants in Anabaena (Cyanobacteria) Leo Rouhiainen, Jouni Jokela, David P. Fewer, Marina Urmann, Kaarina Sivonen Chemistry & Biology Volume 17, Issue 3, Pages 265-273 (March 2010) DOI: 10.1016/j.chembiol.2010.01.017 Copyright © 2010 Elsevier Ltd Terms and Conditions

Figure 1 Biosynthesis of Anabaenopeptins A, B and C on the Peptide Synthetases with Alternative Bimodular Starter Proteins in Anabaena Sp. Strain 90 The pathways using either AptA1 (dashed arrows) or AptA2 (dotted arrows) are shown to yield arginine (B) and lysine (C) or tyrosine (A) anabaenopeptin variants, respectively. The substrate amino acids are loaded in L-form; L-lysine is isomerized to D-lysine by the epimerase domain in AptA1 and in AptA2. It is still unclear at which step the ureido bond is formed. Chemistry & Biology 2010 17, 265-273DOI: (10.1016/j.chembiol.2010.01.017) Copyright © 2010 Elsevier Ltd Terms and Conditions

Figure 2 Anabaenopeptin Synthetase Gene Clusters in Cyanobacteria (A) Anabaenopeptin synthetase gene cluster in Anabaena sp. strain 90. (B) Anabaenopeptin synthetase clusters of Nostoc punctiforme PCC 73102 and Nodularia spumigena CCY9414. Abbreviations for domains: A, adenylation; C, condensation; T, thiolation; E, epimerization; M, N-methyltransferase and Te, thioesterase. Chemistry & Biology 2010 17, 265-273DOI: (10.1016/j.chembiol.2010.01.017) Copyright © 2010 Elsevier Ltd Terms and Conditions

Figure 3 Substrate Specificity of Anabaena Sp Strain 90 anabaenopeptin synthetase adenylation domains by ATP-pyrophosphate exchange assay. Amino acids used as substrates were those found in anabaenopeptins A, B, and C and the chemically related amino acids. Chemistry & Biology 2010 17, 265-273DOI: (10.1016/j.chembiol.2010.01.017) Copyright © 2010 Elsevier Ltd Terms and Conditions