Molecular epidemiology of extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains in a university hospital in Tunis, Tunisia, 1999–2005  D. Elhani, L. Bakir, M. Aouni, V. Passet, G. Arlet, S. Brisse, F.-X. Weill  Clinical Microbiology and Infection  Volume 16, Issue 2, Pages 157-164 (February 2010) DOI: 10.1111/j.1469-0691.2009.03057.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG 1 Evolution of CTX-M-15 and SHV-12 incidence during the study period. Clinical Microbiology and Infection 2010 16, 157-164DOI: (10.1111/j.1469-0691.2009.03057.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG 2 EcoRI restriction profiles of plasmids from transconjugants producing SHV-12 enzymes and Southern hybridization with the blaSHV-12 probe. Lanes: 1, Raoul marker (QBiogene, Illkirch, France); 2, Tc-17 (profile S4); 3, Tc-16 (profile S3); 4, Tc-18 (profile 11); 5, Tc-21 (profile Q1); 6, 1-kb DNA ladder; 7, Tc-8 (profile H4); 8, Tc-20 (profile N1); 9, Tc-19 (profile 11); 10, Tc-4 (profile E3); 11, Tc-2 (profile E2); 12, Tc-13 (profile S1); 13, Tc-1 (profile E1); 14, Raoul marker (QBiogene). For Tc-1, Tc-2, Tc-4, Tc-20 and Tc-16, the presence of multiple positive hybridization signals of the blaSHV-12 gene is due to the presence of several copies of this gene in the same plasmid (Table 1). Clinical Microbiology and Infection 2010 16, 157-164DOI: (10.1111/j.1469-0691.2009.03057.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG 3 PstI restriction profiles of plasmids from transconjugants or electroporants producing CTX-M-15 enzymes and Southern hybridization with the blaCTX-M-15 probe. Lanes: 1, Raoul marker (QBiogene); 2, Tc-48 (profile A3); 3, Tc-46 (profile A1); 4, Tc-45 (profile A4); 5, Ep-36 (profile B5); 6, Tc-41 (profile C1); 7, 1-kb DNA ladder; 8, Tc-42 (profile A3); 9, Ep-34 (profile B4); 10, Ep-33 (profile B3); 11, Ep-37 (profile B2); 12, Ep-35 (profile B1); 13, Tc-43 (profile A2); 14, Raoul marker (QBiogene). The presence of multiple positive hybridization signals of the blaCTX-M-15 gene is probably due to the partial digestion of the plasmid carrying the blaCTX-M15 gene (Tc-43 and Tc-45) or due to the presence of different plasmids in the same preparation (Ep-34) (Table 1). Clinical Microbiology and Infection 2010 16, 157-164DOI: (10.1111/j.1469-0691.2009.03057.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions