AM Campbell, JB Tok, J Zhang, Y Wang, M Stein, DG Lynn, AN Binns

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Xenognosin sensing in virulence: is there a phenol receptor in Agrobacterium tumefaciens? AM Campbell, JB Tok, J Zhang, Y Wang, M Stein, DG Lynn, AN Binns Chemistry & Biology Volume 7, Issue 1, Pages 65-76 (January 2000) DOI: 10.1016/S1074-5521(00)00065-X

Figure 1 Structural designations. Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)

Figure 2 Induction of vir expression. β-Galactosidase expression in A348 carrying pSW209 (virB::lacZ) induced with (a) (−)-trans-DDF, (+)-trans-DDF, and (b) (+)-trans-CP and (−)-trans-CP. Expressed as mean ± standard deviation (n=3). Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)

Figure 3 Cell growth. Kanamycin-resistant growth of wild-type A348 and AB140, each carrying pAC2 (virBP/nptII), expressed as a percentage of control with AS induction in wild-type A348/pAC2, AV in wild-type A348/pAC2, AS in AB140/pAC2, and AV in AB140/pAC2. Expressed as mean ± standard deviation (n=3). Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)

Figure 4 Expression of virB10. Immunoblot analysis of AS induction in (a) wild-type A348 and (b) AB140 with anti-VirB10 antibody following induction with the indicated concentration of AS (in μM). Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)

Figure 5 Tumor induction. Tobacco leaf square pieces were co-cultivated with wild-type A348 and AB140 in the presence of various concentrations of AS. After 12 days, the leaf pieces were scored for the mean number of tumors per leaf square ± standard error (n=20). Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)

Figure 6 Induction of vir expression. β-Galactosidase induction with (a) D-indanone, (b) Z-indanone, and (c) E-indanone in wild-type A348, AB140 and AB144 carrying pSW209 (virB::lacZ). Mean – standard deviation (n=3). Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)

Figure 7 Induction of vir expression. β-Galactosidase induction in strains grown at varying AS concentrations in ABIM plus 1% glycerol in the (a,c) presence or (b,d) absence of 0.1% arabinose. (a,b) A348/pSW209 and AB140/pSW209, (c,d) A348/pSW209 and AB147/pSW209. Mean ± standard deviation (n=3). Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)

Figure 8 Induction of vir expression. Ti plasmids of wild-type A348 and AB140 were conjugated into UIA143/pSW209 and β-galactosidase expression was measured in UIA143/pTiA6wt and UIA143/pTiA6140. Mean ± standard deviation (n=3). Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)

Figure 9 Model of the D-indanone receptor. Regions A, B and C represent domains of the Xbp involved in binding. Chemistry & Biology 2000 7, 65-76DOI: (10.1016/S1074-5521(00)00065-X)