Expression of Glasso network genes in PC12 cells.

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Expression of Glasso network genes in PC12 cells. Expression of Glasso network genes in PC12 cells. Relative mRNA expression of genes in the network was examined in both undifferentiated and NGF-treated PC12 cells by qRT-PCR. The relative mRNA level was calculated using 2^(−ΔΔct) method where the expression level of undifferentiated sample was calculated as 1. Since Pdyn is undetectable at 40 cycles on qRT-PCR in undifferentiated PC12 cells, the relative mRNA level was calculated by assumption of Ct value of undifferentiated sample as 40 cycles. Error bar, SEM; *p < 0.05; **p < 0.01; unpaired Student’s t test; n = 3. Su-Yi Loh et al. eNeuro 2017;4:ENEURO.0243-17.2017 ©2017 by Society for Neuroscience