Oligo#5 influences the ratio of the two serotonin 2C receptor isoforms and changes the localization of the full‐length receptor Oligo#5 influences the.

Slides:

Advertisements

Similar presentations

Supplementary Figure 1. AB Supplementary Figure 1. L1 and Alu retrotransposition assay. A. Schematic of L1 retrotransposition plasmid (L1). ORF1 and ORF2.

Advertisements

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Overexpression of PAI‐1 reverses the arsenite‐mediated effect on senescence Overexpression of PAI‐1 reverses the arsenite‐mediated effect on senescence.

E2F7/8 and HIF1 are required for hypoxic VEGFA expression.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

BRD4 expression levels are associated with sensitivity to BET inhibition in NRAS‐mutant melanoma BRD4 expression levels are associated with sensitivity.

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

The Role of Transcription Factor PU

HIF1 binds and stimulates the VEGFA promoter in normoxia.

The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth A22Rv1.

Non‐specific activation of gene transcription by exogenous Fe65 in luciferase‐based reporter assays. Non‐specific activation of gene transcription by exogenous.

[68Ga]Pentixafor PET imaging of MM xenografts Real‐time PCR analysis of cxcr4 transcript expression levels in HeLa (negative control) and in MM cell lines.

Generation and characterization of Vps15‐null mice and cells. A

Involvement of SR Proteins in mRNA Surveillance

HS induces sustained H3K4me3 and H3K4me2 methylation at HSP22

HPV‐E7 enhances chemotherapy‐mediated cellular stress and CerS1/ceramide‐mediated lethal mitophagy HPV‐E7 enhances chemotherapy‐mediated cellular stress.

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

LGN is essential for Plk1-dependent cortical NuMA enrichment during metaphase, but not in anaphase. LGN is essential for Plk1-dependent cortical NuMA enrichment.

Volume 5, Issue 2, Pages (October 2013)

Exon Circularization Requires Canonical Splice Signals

HPV‐E7/RB axis regulates ceramide‐dependent mitophagy via E2F5 activation HPV‐E7/RB axis regulates ceramide‐dependent mitophagy via E2F5 activation AUM‐SCC‐47.

PEX13 is required for Sindbis virophagy but not general autophagy

Characterization of oligo#5 and its derivatives

TCF19 is required for survival of NRAS‐mutant melanoma cells

Volume 13, Issue 1, Pages (January 2004)

Volume 37, Issue 6, Pages (March 2010)

Effect of HUWE1 on MYC and MIZ1 complexes Immunoblot documenting levels of MYC and MIZ1 upon HUWE1 overexpression. Effect of HUWE1 on MYC and MIZ1 complexes.

CDK6 and FOXO3 regulate ATR expression and protect platinum‐treated cells from premature chromosome condensation (PCC)‏ CDK6 and FOXO3 regulate ATR expression.

BRD4 expression levels are associated with sensitivity to BET inhibition in NRAS‐mutant melanoma BRD4 expression levels are associated with sensitivity.

(A) Schematic representation of full‐length APC

RRNA Modifications in an Intersubunit Bridge of the Ribosome Strongly Affect Both Ribosome Biogenesis and Activity Xue-hai Liang, Qing Liu, Maurille.

Volume 10, Issue 1, Pages (July 2002)

Dynamic map of the Nap1p/Kcc4p interaction.

Transcriptional Termination Enhances Protein Expression in Human Cells

Improved design and localization of mtZFNs Schematic structure of previous mtZFN architecture. Improved design and localization of mtZFNs Schematic structure.

Hdac3 deletion decreases AKT phosphorylation and tumor growth in Pten knockout prostate cancer Hdac3 deletion decreases AKT phosphorylation and tumor growth.

The disease‐linked H157Y variant of TREM2 is shed more rapidly from the cell surface The disease‐linked H157Y variant of TREM2 is shed more rapidly from.

TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

The mutant ZIP13 protein is degraded through a VCP‐dependent mechanism Identification of VCP/Cdc48/p97 as a ZIP13‐associating protein. The mutant ZIP13.

Fmrp regulates E‐cadherin and Vimentin in breast cancer cells Fmrp (red) and E‐cadherin (green) detection by I.F. in 4T1 cells expressing different Fmrp.

ZIP13G64D protein is readily degraded by a proteasome‐dependent mechanism Proteasome inhibitor treatments. 293T cells were transfected with WT‐V5 or G64D‐V5.

HPV‐E7 targets RB for induction of ceramide‐dependent mitophagy

Lack of RAD51 nuclear foci identifies PARPi‐sensitive PDX tumors

R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the.

Soluble TLR4 can block Ni2+/Co2+‐induced IL‐8 production.

C266S laforin's effect on LD‐associated general defect in autophagy

Protein interactions at the nuclear pore.

Identification of a homozygous loss‐of‐function mutation in RASGRP1 in two siblings with Hodgkin lymphoma and defective immunity to EBV Identification.

Oligo#5 enters neurons after ICV injection, changes 5HT2C splicing and food intake Oligo#5 enters neurons after ICV injection, changes 5HT2C splicing and.

TIPI mediates rapid degradation of proteins in yeast.

Protein tyrosine kinase 7 is involved in Wnt/β‐catenin canonical signalling. Protein tyrosine kinase 7 is involved in Wnt/β‐catenin canonical signalling.

Generation and molecular characterization of LV producer cell lines

Drp1 knockdown prevents mitophagy

Both E102Q and L95fs Sig1R variants are unstable and lose their abilities to control Ca2+ flux in Neuro2a cells Both E102Q and L95fs Sig1R variants are.

The CHCH domain is necessary for mitochondrial import of CHCHD10

Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6R cells to PORCN inhibition Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6R.

Ubqln1 interacts with Ubqln4.

Effect of RK‐33 on radiation‐induced DNA damage AImmunofluorescence images showing 53BP1 and γH2AX foci in A549 cells after 2‐Gy radiation and A549 cells.

Single‐cell RT–qPCR data

Cortical NuMA enrichment upon Plk1 inhibition is dynein independent.

DIA1(R1204X) induces elongated and thick microvilli in HeLa cells

Mpdz was localized proximal to the apical surface of CPECs

Plk1 inhibition affects the NuMA turnover at the spindle pole.

RIF1 interacts with protein phosphatase 1 isoforms

Maria Paola Paronetto, Belén Miñana, Juan Valcárcel Molecular Cell

CRISPR‐Cas9 gene perturbation profiling in HeLa cells

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Modification of the β-tubulin C-terminal tail does not affect the partitioning of tubulin between the soluble and polymerized fractions in response to.

Fig. 4 Cdc42 enhances the cellular uptake of EVs.

Nonsense-Associated Altered Splicing

Presentation transcript:

Oligo#5 influences the ratio of the two serotonin 2C receptor isoforms and changes the localization of the full‐length receptor Oligo#5 influences the ratio of the two serotonin 2C receptor isoforms and changes the localization of the full‐length receptor AStructure of the reporter gene introduced into cells. The start codon in exon III is shown (circle) and the splicing patterns emerging from using the distal and proximal splice site (DS, PS). Skipping of exon Vb causes a frameshift, which terminates the protein in the first stop codon (square). Exon Vb inclusion keeps a longer open reading frame that terminates after the GFP sequence. The encoded proteins are schematically shown below.BEffect of oligo#5 on pre‐mRNA splicing of the reporter gene, stably transfected into HeLa cells.CEffect of oligo#5 on the truncated 5HT2C protein. Stable cell lines expressing the construct shown in panel (A) were treated with oligo#3 and oligo#5. After 48 h, cells were separated in membrane‐containing fractions and soluble cytosolic supernatant. The fractions were analyzed by Western blot using the anti‐RNA1 antiserum (Fig EV1). The protein samples were on the same membrane, but the supernatant fractions were exposed about ten times longer.DEffect of oligo#5 on the full‐length 5HT2C protein. The protein was prepared as in panel (C) and analyzed using a polyclonal anti‐GFP antiserum.EQuantification of panels (C) and (D) from three independent experiments. The ratio of protein signal after oligo#3 and oligo#5 treatment is shown for RNA1 (*P = 0.002) and RNA2 (***P = 0.0003) (Student's t‐test), n = 3, respectively. Error bars indicate standard deviation.F, GEffect of oligo#5 on localization of the EGFP‐tagged serotonin 2C receptor. Confocal images of three representative pictures are shown.HQuantification of GFP signal found on the membrane after oligo#5 addition. The signal around the peripheral region was determined by visual inspection and calculated as a fraction of total signal using NIH image. Two hundred cells from three individual experiments were analyzed, **P < 0.008 (Student's t‐test), n = 200. Error bars indicate standard deviation. Source data are available online for this figure. Zhaiyi Zhang et al. EMBO Mol Med. 2016;8:878-894 © as stated in the article, figure or figure legend