Prevascularization with gelatin microspheres containing basic fibroblast growth factor enhances the benefits of cardiomyocyte transplantation Yutaka.

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Prevascularization with gelatin microspheres containing basic fibroblast growth factor enhances the benefits of cardiomyocyte transplantation Yutaka Sakakibara, MDa, Kazunobu Nishimura, MD, PhDa, Keiichi Tambara, MDa, Masaya Yamamoto, PhDb, Fanglin Lu, MDa, Yasuhiko Tabata, PhDb, Masashi Komeda, MD, PhDa The Journal of Thoracic and Cardiovascular Surgery Volume 124, Issue 1, Pages 50-56 (July 2002) DOI: 10.1067/mtc.2002.121293 Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 1 Experimental protocol. 1st Op, Ligation of the left anterior descending coronary artery; Treatment I, culture medium injection or fetal cardiomyocyte transplantation or injection of gelatin hydrogel microspheres incorporating bFGF (bFGF microsphere injection); Treatment II, bFGF microsphere injection followed by fetal cardiomyocyte transplantation; Echo, echocardiography; Cath, measurement of the LV pressure with a micromanometer-tipped catheter; and Hist, histologic study. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 50-56DOI: (10.1067/mtc.2002.121293) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 2 In vivo release of bFGF and gelatin hydrogels. Patterns of declining radioactivity in the back subcutis of mice after subcutaneous implantation of 125I-labeled bFGF in solution (open triangles), in hydrogels (open circles), and in 125I-labeled gelatin hydrogels (filled circles). The Journal of Thoracic and Cardiovascular Surgery 2002 124, 50-56DOI: (10.1067/mtc.2002.121293) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 3 Indices of LV maximum time-varying elastance (LVEmax), LV end-diastolic pressure (LVEDP), and τ in hearts of mice from the control, TX, FGF, and FGF-TX groups 4 weeks after each treatment. Error bars show SEM. The FGF-TX group shows a higher LVEmax than the other groups (*P <.05 and **P <.01 vs FGF-TX group). The FGF-TX group shows a lower LVEDP than the other groups (†P <.05 vs control group). There was no significant difference in τ among the 4 groups. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 50-56DOI: (10.1067/mtc.2002.121293) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 4 A, Neovascularization in the scar tissue of the left ventricle free wall 1 week after the injection of bFGF microspheres. Arrow indicates neovessels. (Hematoxylin and eosin, original magnification 100×.) B, Capillary density in scar and peri-infarct area in each treatment group (n = 5) is expressed as the number of capillary vessels per high-power field. Data are shown as means ± SEM. The bFGF treatment groups have a higher capillary vessel density than the control group in both areas (*P <.001). The Journal of Thoracic and Cardiovascular Surgery 2002 124, 50-56DOI: (10.1067/mtc.2002.121293) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 5 A-C, Histologic findings 4 weeks after each treatment: A, control group; B, TX group; C, FGF-TX group. (Hematoxylin and eosin staining, original magnification 1×.) D and E, Fluorescent image of transplanted cells labeled with PKH26 red fluorescent dye (D, peri-infarct area in the TX group; E, middle of the myocardial infarction in the FGF-TX group). (Original magnification 40×.) The Journal of Thoracic and Cardiovascular Surgery 2002 124, 50-56DOI: (10.1067/mtc.2002.121293) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions