Detection of a new subgenotype of hepatitis B virus genotype A in Cameroon but not in neighbouring Nigeria J.M. Hübschen, P.O. Mbah, J.C. Forbi, J.A.

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Detection of a new subgenotype of hepatitis B virus genotype A in Cameroon but not in neighbouring Nigeria J.M. Hübschen, P.O. Mbah, J.C. Forbi, J.A. Otegbayo, C.M. Olinger, E. Charpentier, C.P. Muller Clinical Microbiology and Infection Volume 17, Issue 1, Pages 88-94 (January 2011) DOI: 10.1111/j.1469-0691.2010.03205.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Genotype E (a) and A cluster (b) of a phylogenetic tree based on 815 nucleotides of the S fragment of sequences obtained during the present study as well as reference sequences of genotypes A-J (Neighbour-joining algorithm using the Kimura two-parameter model). Symbols designate the country of origin for strains: Nigeria (•), Cameroon (Δ) and Rwanda (♦). Only single strains of sequences from the same cohort with identical clustering are shown (18, 9, 5, 4 and 3 strains grouped similar to 42126, 25004, 42002, 25135 and 42334, respectively; two strains each grouped identical to 25060, 36048 and 42203; one strain each identical to 36135, 36083, 29070, 29085, 36062, 25044 and 49037). In (a), clusters containing only sequences from Nigeria are represented by triangles; clusters containing both sequences from Cameroon and Nigeria are shown in full detail. Clinical Microbiology and Infection 2011 17, 88-94DOI: (10.1111/j.1469-0691.2010.03205.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Phylogenetic tree based on complete genome sequences (Neighbour-joining algorithm using the Kimura two-parameter model). Symbols designate the country of origin for strains: Nigeria (•) and Cameroon (Δ). Seventeen and six strains from the same cohorts grouping as strains 42128 and 42002, respectively, are not shown. Clinical Microbiology and Infection 2011 17, 88-94DOI: (10.1111/j.1469-0691.2010.03205.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Phylogenetic trees based on the S gene (a), the preC/C region (b) and the preS region (c) of the hepatitis B virus genome (Neighbour-joining algorithm using the Kimura two-parameter model). Symbols designate the new variant strains (Δ). Clinical Microbiology and Infection 2011 17, 88-94DOI: (10.1111/j.1469-0691.2010.03205.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions