Monoclonality of Intraepidermal T Lymphocytes in Early Mycosis Fungoides Detected by Molecular Analysis after Laser-Beam-Based Microdissection  Lorenzo Cerroni, Edith Arzberger, Marco Ardigò, Barbara Pütz, Helmut Kerl  Journal of Investigative Dermatology  Volume 114, Issue 6, Pages 1154-1157 (June 2000) DOI: 10.1046/j.1523-1747.2000.00984.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Early mycosis fungoides. (a) Early patch of mycosis fungoides showing intraepidermal lymphocytes and a scarce dermal infiltrate; (b) detail showing intraepidermal lymphocytes without so-called Pautrier's collections. Journal of Investigative Dermatology 2000 114, 1154-1157DOI: (10.1046/j.1523-1747.2000.00984.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Skin biopsy specimen after preparation with a laser beam. Epidermis and dermis are separated completely before microdissection. Journal of Investigative Dermatology 2000 114, 1154-1157DOI: (10.1046/j.1523-1747.2000.00984.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Results of molecular analysis of TCR-γ gene rearrangement in MF cases. Lane 1, molecular ladder; lanes 2–16, epidermal samples, dermal samples, and samples containing the entire specimen (2, 5, 8, 11, 14, epidermal samples, cases 1–5; 3, 6, 9, 12, 15, dermal samples, cases 1–5; 4, 7, 10, 13, 16, entire specimen, cases 1–5); lane 17, negative control (no DNA); lane 18, positive control (CTCL). Journal of Investigative Dermatology 2000 114, 1154-1157DOI: (10.1046/j.1523-1747.2000.00984.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Results of molecular analysis of TCR-γ gene rearrangement in control cases. Lanes 1–3, epidermal sample, dermal sample, and sample containing the entire specimen (pityriasis lichenoides et varioliformis acuta); lanes 4–6, epidermal sample, dermal sample, and sample containing the entire specimen (lichen planus-like keratosis); lanes 7–9, epidermal sample, dermal sample, and sample containing the entire specimen (contact dermatitis); lanes 10–12, epidermal sample, dermal sample, and sample containing the entire specimen (inflamed seborrheic keratosis); lane 13, negative control (no DNA); lane 14, positive control (CTCL); lane 15, molecular ladder. Journal of Investigative Dermatology 2000 114, 1154-1157DOI: (10.1046/j.1523-1747.2000.00984.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions