Mutation and Abnormal Expression of the p53 Gene in the Viral Skin Carcinogenesis of Epidermodysplasia Verruciformis Kamila. Padlewska, Nicolas. Ramoz,

Slides:

Advertisements

Similar presentations

Skin-Specific Expression of ank-393, a Novel Ankyrin-3 Splice Variant

Advertisements

Pooja Chitgopeker, Debjani Sahni Journal of Investigative Dermatology

Cohort Studies (and Skin Cancer) Never Come Alone

Basement Membrane Zone Remodeling During Appendageal Development in Human Fetal Skin. The Absence of Type VII Collagen is Associated with Gelatinase-A.

DMBA/TPA Treatment Is Necessary for BCC Formation from Patched Deficient Epidermal Cells in Ptchflox/floxCD4Cre+/− Mice Anja Uhmann, Ina Heß, Anke Frommhold,

Marguerite Stas, Hugo Degreef Journal of Investigative Dermatology

Differential Modulation of Ku70/80 DNA-Binding Activity in a Patient with Multiple Basal Cell Carcinomas Paola Mazzarelli, Carla Rabitti, Paola Parrella,

Epidermal and Hair Follicle Progenitor Cells Express Melanoma-Associated Chondroitin Sulfate Proteoglycan Core Protein Lucy Ghali, Soon-Tee Wong, Nick.

Interferon α-Inducible Protein 27 (IFI27) is Upregulated in Psoriatic Skin and Certain Epithelial Cancers Sari Suomela, Li Cao, Anne Bowcock, Ulpu Saarialho-Kere,

Osteopontin Gene is Expressed in the Dermal Papilla of Pelage Follicles in a Hair- Cycle-Dependent Manner Tian Yang, Pamela J. Jensen, Robert M. Lavker

Human Papillomavirus Gene Expression in Cutaneous Squamous Cell Carcinomas from Immunosuppressed and Immunocompetent Individuals Karin J. Purdie, Thirunavukarasu.

Novel Homozygous and Compound Heterozygous COL17A1 Mutations Associated with Junctional Epidermolysis Bullosa Michaela Floeth, Heike Schäcke, Nadja Hammami-Hauasli,

High β-HPV DNA Loads and Strong Seroreactivity Are Present in Epidermodysplasia Verruciformis Valentina Dell'Oste, Barbara Azzimonti, Marco De Andrea,

High Frequency of Loss of Heterozygosity on Chromosome Region 9p21–p22 but Lack of p16INK4a/p19ARF Mutations in Greek Patients with Basal Cell Carcinoma.

Detection of Expanded T Cell Clones in Skin Biopsy Samples of Patients with Lichen Sclerosus et Atrophicus by T Cell Receptor-γ Polymerase Chain Reaction.

The Complexity of Elastic Fiber Biogenesis: The Paradigm of Cutis Laxa

Hotspot Mutation of Brahma in Non-Melanoma Skin Cancer

Isolation and Characterization of a Putative Keratin-Associated Protein Gene Expressed in Embryonic Skin of Mice Mikiro Takaishi, Yoshimi Takata, Toshio.

Matthew A. Sleeman, James D. Watson, J. Greg Murison

A Possible Vertical Transmission of Human Papillomavirus Genotypes Associated with Epidermodysplasia Verruciformis Michel Favre, Slavomir Majewski, Nando.

Histidine Decarboxylase Expression in Human Melanoma

Presence of Epstein–Barr Virus in Langerhans Cells of CTCL Lesions

Ute Breitenbach, Jan P. Tuckermann, Christoffer Gebhardt, Karl H

Μ-Crystallin, Thyroid Hormone-binding Protein, is Expressed Abundantly in the Murine Inner Root Sheath Cells Noriaki Aoki, Kaoru Ito, Masaaki Ito Journal.

Olga M. Mazina, Marjorie A. Phillips, Trevor Williams, Carol A

Inherited Junctional Epidermolysis Bullosa in the German Pointer: Establishment of a Large Animal Model Annabelle Capt, Flavia Spirito, Eric Guaguere,

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Transduction of the E6 and E7 Genes of Epidermodysplasia-Verruciformis-Associated Human Papillomaviruses Alters Human Keratinocyte Growth and Differentiation.

Mutant Loricrin is Not Crosslinked into the Cornified Cell Envelope but is Translocated into the Nucleus in Loricrin Keratoderma Akemi Ishida-Yamamoto,

Francois le Pelletier, Anne Janin Journal of Investigative Dermatology

P16INK4a and p14ARF Tumor Suppressor Genes Are Commonly Inactivated in Cutaneous Squamous Cell Carcinoma Victoria L. Brown, Catherine A. Harwood, Tim.

Cancer Cell Expression of Urokinase-Type Plasminogen Activator Receptor mRNA in Squamous Cell Carcinomas of the Skin John Rømer, Charles Pyke, Leif R.

The Majority of Keratinocytes Incorporate Intradermally Injected Plasmid DNA Regardless of Size but Only a Small Proportion of Cells Can Express the Gene.

Response of Psoriasis to Interleukin-10 is Associated with Suppression of Cutaneous Type 1 Inflammation, Downregulation of the Epidermal Interleukin-8/CXCR2.

Efficient Expression of Naked Plasmid DNA in Mucosal Epithelium: Prospective for the Treatment of Skin Lesions Ulrich R. Hengge Journal of Investigative.

A Novel Homozygous Mutation Affecting Integrin α6 in a Case of Junctional Epidermolysis Bullosa with Pyloric Atresia Detected In Utero by Ultrasound Examination

Eric J. Stelnicki, László G. Kömüves, Angela O

Scott B. Shappell, Jing Zhang, Robert Page, Sandy J. Olson

A Transposon-Based Analysis of Gene Mutations Related to Skin Cancer Development Rita M. Quintana, Adam J. Dupuy, Ana Bravo, M Llanos Casanova, Josefa.

Lack of EVER2 Protein in Two Epidermodysplasia Verruciformis Patients with Skin Cancer Presenting Previously Unreported Homozygous Genetic Deletions in.

Tazarotene-Induced Gene 3 Is Suppressed in Basal Cell Carcinomas and Reversed In Vivo by Tazarotene Application Madeleine Duvic, Xiao Ni, Rakhashandra.

Role of Cytotoxic T Cells in Chronic Alopecia Areata

Carol S. Trempus, John E. French, Raymond W. Tennant

Multiple Epidermal Connexins are Expressed in Different Keratinocyte Subpopulations Including Connexin 31 Wei-Li Di, Elizabeth L. Rugg, Irene M. Leigh,

BRAF and NRAS Mutations Are Frequent in Nodular Melanoma but Are not Associated with Tumor Cell Proliferation or Patient Survival Lars A. Akslen, Sabrina.

Presence of Chimeric Maternally Derived Keratinocytes in Cutaneous Inflammatory Diseases of Children: The Example of Pityriasis Lichenoides Kiarash Khosrotehrani,

Human Papillomavirus Infection and Skin Cancer Risk in Organ Transplant Recipients Jan N. Bouwes Bavinck, Mariet Feltkamp, Linda Struijk, Jan ter Schegget

Juliette Lois Lee, Arianna Kim, Levy Kopelovich, David R

Expression of the CD4+ Cell-Specific Chemoattractant Interleukin-16 in Mycosis Fungoides Volker Blaschke, Kristian Reich, Michael Letschert, Florian.

Ramine Parsa, Annie Yang, Frank McKeon, Howard Green

Wook Lew Journal of Investigative Dermatology

Matrilysin-2 (Matrix Metalloproteinase-26) Is Upregulated in Keratinocytes During Wound Repair and Early Skin Carcinogenesis Katja Ahokas, Tiina Skoog,

Ultraviolet Light Downregulates CD95 Ligand and Trail Receptor Expression Facilitating Actinic Keratosis and Squamous Cell Carcinoma Formation Felix.

Expression of Mast Cell Growth Modulating and Chemotactic Factors and their Receptors in Human Cutaneous Scars Barbara Hermes Journal of Investigative.

Research Snippets from the British Journal of Dermatology

Single Base Instability Is Promoted in Vulvar Lichen Sclerosus

M2 Muscarinic Acetylcholine Receptor (mAchR) Subtype Is Present in Human Epidermal Keratinocytes In Situ and In Vitro S.M.A. Elwary, S. Hasse, K.U. Schallreuter

Immunoprotective UVA (320–400 nm) Irradiation Upregulates Heme Oxygenase-1 in the Dermis and Epidermis of Hairless Mouse Skin Munif Allanson, Vivienne.

A Lack of Birbeck Granules in Langerhans Cells Is Associated with a Naturally Occurring Point Mutation in the Human Langerin Gene Pauline Verdijk, Remco.

The Level of Prosaposin is Decreased in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Silke Stachowitz, Johannes Ring, Heidrun.

Gli1 Protein is Expressed in Basal Cell Carcinomas, Outer Root Sheath Keratinocytes and a Subpopulation of Mesenchymal Cells in Normal Human Skin Lucy.

Differences in Imiquimod Treatment Outcome in Two Patients with Bowenoid Papulosis Containing Either Episomal or Integrated Human Papillomavirus 16 Georges.

BRAF Mutations Are Common Somatic Events in Melanocytic Nevi1

SLURP1 Is a Late Marker of Epidermal Differentiation and Is Absent in Mal de Meleda Bertrand Favre, Laure Plantard, Lorène Aeschbach, Noureddine Brakch,

Gonosomal Mosaicism for a Nonsense Mutation (R1947X) in the NF1 Gene in Segmental Neurofibromatosis Type 1 Claudia Consoli, Celia Moss, Stuart Green,

Loss of Keratin 10 Leads to Mitogen-activated Protein Kinase (MAPK) Activation, Increased Keratinocyte Turnover, and Decreased Tumor Formation in Mice

David A. Norris, Brian L. Kotzin Journal of Investigative Dermatology

Journal of Investigative Dermatology

Tsutomu Soma, Cord E. Dohrmann, Toshihiko Hibino, Laurel A. Raftery

Matrix Metalloproteinase Inhibitor BB-3103 Unlike the Serine Proteinase Inhibitor Aprotinin Abrogates Epidermal Healing of Human Skin Wounds Ex Vivo1

Presentation transcript:

Mutation and Abnormal Expression of the p53 Gene in the Viral Skin Carcinogenesis of Epidermodysplasia Verruciformis Kamila. Padlewska, Nicolas. Ramoz, Patricia. Cassonnet, Guy. Riou, Michel. Barrois, Slavomir. Majewski, Odile. Croissant, Stefania. Jablonska, Gérard. Orth Journal of Investigative Dermatology Volume 117, Issue 4, Pages 935-942 (October 2001) DOI: 10.1046/j.0022-202x.2001.01515.x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 SSCP analysis of amplified p53 exons from EV biopsy specimens. Exons 5 through 8 were PCR amplified in the presence of [α33P]-dCTP. PCR products were heat-denatured and single-stranded DNA molecules were fractionated on nondenaturing polyacrylamide gels and detected by autoradiography as described in Materials and Methods. Capital and lower case letters given on the top of the lanes correspond to the patients and specimens described in Table I. Aberrant p53 DNA migration patterns (+) show a band or bands with altered migration compared with the wild-type p53 DNA migration patterns (–). Journal of Investigative Dermatology 2001 117, 935-942DOI: (10.1046/j.0022-202x.2001.01515.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 In situ detection of HPV5 DNA in lesions with a mutated p53 gene. Sections of an AK (A; specimen A, a) and a CIS (B; specimen A, f) were hybridized with a tritiated HPV5 DNA probe. Hybrids are detected in the nuclei of upper differentiating cells, where vegetative viral DNA replication occurs (Orth et al, 1980), and in differentiated cells. Scale bar: 100 µm. Journal of Investigative Dermatology 2001 117, 935-942DOI: (10.1046/j.0022-202x.2001.01515.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Positive p53 immunostaining of lesions from two EV patients. Six of the EV specimens described in Table I are illustrated. (A) Benign EV macular lesion (A, i) with a nuclear staining confined to basal cells; (B) CIS adjacent to a positive SCC (A, n) with a labeling sparing the superficial differentiating cells; (C) AK (B, a) with a staining of almost all immature keratinocytes and lack of staining of a hair follicle; (D), (E) microinvasive (A, m) and invasive (B, d) SCC with a strong staining of almost all undifferentiated cells; (F), (G) consecutive sections of a BCC (A, p) showing a congruent moderate p53 immunoreactivity and strong Bcl-2 immunostaining. Scale bar: 30 µm. Journal of Investigative Dermatology 2001 117, 935-942DOI: (10.1046/j.0022-202x.2001.01515.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions