Side-view and apical-view projections of mutant and validation strain biofilms. Side-view and apical-view projections of mutant and validation strain biofilms.

Slides:

Advertisements

Similar presentations

Phenotypic analysis of spo73∆ mutant.

Advertisements

Validation of Tn-seq fitness defects in rich and defined media.

MFS1 expression in IPO323 MFS1 replacement mutants.

ΔsimA yeast cells appear degraded in vivo.

Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ.

H-NS2 is upregulated in an hns mutant derivative of the strain E

Putative ammonia transporters are not required for ammonia release during N-acetylglucosamine pH neutralization. Putative ammonia transporters are not.

Dibutyryl cAMP does not rescue staurosporine-induced filamentation of a cyr1Δ/cyr1Δ mutant. Dibutyryl cAMP does not rescue staurosporine-induced filamentation.

Growth characteristics of strain 22A and its MDH mutant derivatives.

PM analysis of wild-type E. coli K-12 and its rpoZ-defective mutant.

Spores of C. difficile imaged by TEM and SEM

TEM on spore section of strain ATCC 9714.

Ions representative of bronchial tissue (m/z 88

Mutants impaired in N-acetylglucosamine transport and catabolism display morphological and pH neutralization defects within macrophages. Mutants impaired.

Analysis of GPCR deletion strains.

Staurosporine-induced filamentation requires Cyr1 and PKA

The ΔsimA strain shows less cellular proliferation during macrophage infection and ruptured yeast cells. The ΔsimA strain shows less cellular proliferation.

The pho4Δ mutant is less virulent than the WT strain in intranasal (A to C) and intravenous (D to F) models of cryptococcosis. The pho4Δ mutant is less.

IVA can localize to and encase the forespore in the absence of VM

Quantification of drug cross-resistance between mutants selected for I-BET151 resistance and F0002 resistance. Quantification of drug cross-resistance.

Cell wall hydrolytic activities of Andhra lysins.

Direct role of HIS-24 and HPL in stress response.

C. jejuni flaC mutants induce increased cell activation in human and chicken cells. C. jejuni flaC mutants induce increased cell activation in human and.

RNA-FISH validation of male and female markers.

Prenylation and postprocessing enzymes Ram1, Rce1, Ste24, and Ste14 play a role in mating filament formation during C. neoformans sexual development. Prenylation.

Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant with Triton X-100. Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant.

Detection of BapA in envelope fractions of P. denitrificans.

Southern blot analysis of ΔpksP mutant generated in the Af293 background. Southern blot analysis of ΔpksP mutant generated in the Af293 background. (A)

RNA-Seq analysis of CYR1 cells in the adaptation to acid pH.

AAF/II does not mask colonization-associated phenotypes produced by Hra1. AAF/II does not mask colonization-associated phenotypes produced by Hra1. (A)

SsC6TF1 (Sscle04g036970) regulates sclerotial development and virulence on pea. SsC6TF1 (Sscle04g036970) regulates sclerotial development and virulence.

Dynamic ranges of anti-PnPS IgG Reference standard serum 007sp for each of 13 serotypes. Dynamic ranges of anti-PnPS IgG Reference standard serum 007sp.

Phenotypes of Arabidopsis msc mutants.

Developmental defects in the svkA-null mutant.

Loss of Arp2/3 or SCAR activity does not modify α-Cat phenotype during oogenesis. Loss of Arp2/3 or SCAR activity does not modify α-Cat phenotype during.

Growth of Salmonella and E. coli in egg white.

Defective organ development in mab1 mutants.

Growth, adhesion, biofilm formation, and aggregation of WT R2866 and the R2866ΔampG gene deletion mutant. Growth, adhesion, biofilm formation, and aggregation.

TEM observation of the bacterial damage caused by egg white.

E. coli CFT073 and E. coli MG1655 persistence.

Gallium isotopes at m/z and 70

Proteus mirabilis strain HI4320 (WT) and isogenic mutants for dsdA, dsdC, or dsdX were cultured in LB broth overnight and diluted 1:100 into fresh LB (A),

Strategy for marker recycling through CRISPR-Cas9-induced marker excision. Strategy for marker recycling through CRISPR-Cas9-induced marker excision. Consider.

Expression of all the GAP genes is under control of the Csy1 sensor and regulated by nitrogen sources. Expression of all the GAP genes is under control.

Susceptibility to hydrogen peroxide.

SusG LES is required for efficient packing into OMVs

TbERP silencing has minimal effect on proliferation.

SAM transport by Gap4 in S. cerevisiae cells.

Uptake of amino acids by C. albicans.

Therapeutic effect of IL-12/ms treatment on gonococcal infection depends on both IFN-γ and B cells. Therapeutic effect of IL-12/ms treatment on gonococcal.

Phenotypic characterization of low-Cu-responsive transcription factor Mac1 in A. nidulans. Phenotypic characterization of low-Cu-responsive transcription.

Distribution of signaling molecules in a section of P

Stress resistance comparisons.

Septin ring formation and chitin-containing septum formation are aberrant in filaments formed in response to staurosporine. Septin ring formation and chitin-containing.

MMEJ plays a dominant role in double-strand DNA break repair in L

CRISPRi-based repression of the essential gene HSP90 in C. albicans.

CT production profiles of single-SNP strains and double-, triple-, and quadruple-SNP combination strains. CT production profiles of single-SNP strains.

TDIF-Sensitive Xylem Formation in wox4 Mutants

OMVs displaying WT SusG can rescue a strain with a ΔsusG growth phenotype on starch as the carbon source. OMVs displaying WT SusG can rescue a strain with.

Mammalian cell cytotoxicity shown by macrophage response to DM262.

Characteristics of ADs from S. cerevisiae S288C.

Mig1 influences the TOR and cell wall remodeling pathways.

CRISPRi repression with dCas9-repressor fusion constructs.

DNase and proteinase K treatment of WT R2866 and R2866ΔampG gene deletion mutant biofilms. DNase and proteinase K treatment of WT R2866 and R2866ΔampG.

Distribution of selected traits across the >4,000 strains in the most recent version of the database, including cell shape (A), spore formation (B), motility.

Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia. Growth with N-acetylglucosamine rapidly raises the.

DNSP16 mutant MERS-CoV infection produces minimal changes in early host responses. dNSP16 mutant MERS-CoV infection produces minimal changes in early host.

C. neoformans encodes a single family 2 DHODH required for wild-type growth. C. neoformans encodes a single family 2 DHODH required for wild-type growth.

GBM 112 and GBM 144 cells infected in vitro with HCMV resist growth inhibition by temozolomide. GBM 112 and GBM 144 cells infected in vitro with HCMV resist.

Identification of bacterial species producing the anti-Candida molecules. Identification of bacterial species producing the anti-Candida molecules. (A)

Presentation transcript:

Side-view and apical-view projections of mutant and validation strain biofilms. Side-view and apical-view projections of mutant and validation strain biofilms. The WT SN250 strain, ume6Δ/Δ mutant, brg1Δ/Δ mutant, ume6Δ/Δ brg1Δ/Δ double mutant, and UME6:BRG1 validation strains were assayed for phenotypic rescue of biofilm formation. (A) Lateral projections of C. albicans WT, mutant, and validation strain biofilms. A wild-type strain forms a large robust biofilm, while the ume6Δ/Δ, brg1Δ/Δ, and ume6Δ/Δ brg1Δ/Δ mutant strains form defective biofilms. The UME6:BRG1 validation strain forms a biofilm with more than half the thickness of the WT biofilm. (B) Apical-view projections of representative sections of each biofilm. A wild-type strain forms a robustly filamentous biofilm, whereas ume6Δ/Δ, brg1Δ/Δ, and ume6Δ/Δ brg1Δ/Δ mutant strains form biofilms with largely reduced filamentation. The UME6:BRG1 strain forms a biofilm with visibly filamentous cells. Manning Y. Huang et al. mSphere 2018; doi:10.1128/mSphere.00169-18