Supplemental Figure 1 A GENE chr location (hg18) SNP sample ID TLR2

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Supplemental Figure 1 A GENE chr location (hg18) SNP sample ID TLR2 chr4 154845515 CCC-CTC WBM40 154845415 AGC-CGC WBM51 154844159 TTT-TCT WBM57 TLR4 chr9 119515069 TGC-TAC WBM69 B C D #55 E no agonist PAM2CSK4 GFP WT F217S GFP WT F217S p-p38 b-actin Figure S1. Mutational analysis of TLRs in MDS. (a) Identification of three rare SNPs of TLR1 and one rare SNP of TLR4 by captured deep sequencing in MDS BM-MNCs. (b) The presence of TLR2-F217S in both BM-MNC and BM-CD3+ cells of one case of MDS. (c) Lack of TLR2-F217S in BM-MNC or BM-CD3+ cells of another case of MDS. (d) Luciferase analysis of activation of AP1 by WT and F217S TLR2 in transfected 293T cells. Cells were exposed to either no agonist or to PAM2CSK4. (e) Western blot characterization of p38 phosphorylation in 293T cells transfected with either WT or F217S TLR2 and treated with no agonist, MALP2 or PAM2CSK4. Top, Western blot analysis of p-p38; Bottom, Quantification of p-p38 band densities.