A New Mouse Model of Junctional Epidermolysis Bullosa: The LAMB3 628G>A Knockin Mouse Johanna Hammersen, Jin Hou, Stephanie Wünsche, Sven Brenner, Thomas.

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Presentation transcript:

A New Mouse Model of Junctional Epidermolysis Bullosa: The LAMB3 628G>A Knockin Mouse Johanna Hammersen, Jin Hou, Stephanie Wünsche, Sven Brenner, Thomas Winkler, Holm Schneider Journal of Investigative Dermatology Volume 135, Issue 3, Pages 921-924 (March 2015) DOI: 10.1038/jid.2014.466 Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Phenotypic and molecular characteristics of LAMB3 628G>A knockin mice. (a) Homozygous LAMB3 628G>A knockin mouse at the age of 30 hours. Arrows indicate blistering. (b) Hematoxylin and eosin stained sections of skin (top row) and oral mucous membranes (bottom row) of homozygous knockin and healthy control mice. (c) Schematic view of the LAMB3 complementary DNA (cDNA) portion investigated. (d) Analysis of LAMB3 expression in wild-type (wt), heterozygous, and homozygous knockin (ki) mice. Quantitative PCR was performed with three independent cDNA samples of each genotype. Data were normalized to porphobilinogen deaminase gene expression. The graph displays mean±SD. (e) PCR analysis of cDNA exons 6–9 (primers depicted above). (f) Electropherogram of the exon 6/7 intersection in the PCR products displayed in e. (g) Immunofluorescence analysis of skin sections of healthy control (left) and homozygous knockin mice (right) with antibodies recognizing the laminin-332 α3 and β3 chains or collagen VII (bar = 50 μm). Journal of Investigative Dermatology 2015 135, 921-924DOI: (10.1038/jid.2014.466) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions