Extensive growth potential of recessive dystrophic epidermolysis bullosa (RDEB) epidermal keratinocytes Keratinocytes were isolated from the skin of a.

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Extensive growth potential of recessive dystrophic epidermolysis bullosa (RDEB) epidermal keratinocytes Keratinocytes were isolated from the skin of a 4‐year‐old patient with severe‐generalised RDEB linked to homozygous insertion–deletion in COL7A1 (Hilal et al, 1993). Extensive growth potential of recessive dystrophic epidermolysis bullosa (RDEB) epidermal keratinocytes Keratinocytes were isolated from the skin of a 4‐year‐old patient with severe‐generalised RDEB linked to homozygous insertion–deletion in COL7A1 (Hilal et al, 1993). Cultured RDEB cells (blue line) were serially passaged for more than 4 months, displaying a growth potential similar to non‐diseased control cells (YF29) isolated from the foreskin of a newborn (black line). To calculate the percentage of growing colonies, 100 to 1,000 cells were plated into indicator dishes at each passage. Cells were grown for 12 days, fixed and stained with rhodamine B. Colonies were scored as growing or aborted (Barrandon & Green, 1987). Clonal analysis demonstrated the presence of stem cells (holoclones) in a passage VII RDEB culture (95% of growing colonies). Stéphanie Droz‐Georget Lathion et al. EMBO Mol Med. 2015;emmm.201404353 © as stated in the article, figure or figure legend