Overexpressed β-Catenin Localizes to Plasma Membrane in Respiratory Papillomas  Alexandra V. Lucs, Allan L. Abramson, Bettie M. Steinberg  Journal of Investigative Dermatology  Volume 134, Issue 6, Pages 1760-1763 (June 2014) DOI: 10.1038/jid.2014.48 Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 β-Catenin localizes to the plasma membrane in respiratory papillomas. (a) Papilloma and clinically normal tissue from the same patient were stained for β-catenin (red) and E-cadherin (green). 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (blue) was used to stain the nucleus. Scale bars=20 μm. (b) Representative western blots of biopsies of clinically normal tissue (CN) and papilloma tissue (P) from recurrent respiratory papillomatosis (RRP) patients. (c) Relative β-catenin mRNA levels normalized to GAPDH. (d, e) Western blots of phospho-glycogen synthase kinase 3β (GSK-3β) and protein kinase G (PKG), respectively. N indicates normal samples from a non-RRP patient. Donors of the tissues were numbered sequentially. CN6a and CN6b were clinically normal tissues from two sites in the airway of patient 6. Actin was used as a loading control. Journal of Investigative Dermatology 2014 134, 1760-1763DOI: (10.1038/jid.2014.48) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 α-Catenin and actin are mislocalized in recurrent respiratory papillomatosis (RRP). Immunofluorescent images of papilloma and clinically normal tissue from the same patient stained for β-actin (green) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) to stain the nucleus (blue) (a) or α-catenin (red) and E-cadherin (green) (b). Scale bars=5 μm. Journal of Investigative Dermatology 2014 134, 1760-1763DOI: (10.1038/jid.2014.48) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions