DN-Cul3 suppresses transformation and tumor growth through its effects on neurofibromin. DN-Cul3 suppresses transformation and tumor growth through its.

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DN-Cul3 suppresses transformation and tumor growth through its effects on neurofibromin. DN-Cul3 suppresses transformation and tumor growth through its effects on neurofibromin. A, relative differences in soft agar colony growth of NF1-WT (U87, Gli36, SF539) or NF1-null (LN319) glioblastoma cells expressing DN-Cul3 or an inert mutant (L52AE55A) control (DN-Cul3mut). Data are represented as mean ± SD of triplicate measurements. P values shown in the panel were calculated by Student t test. n.s., not statistically significant; WT, wild-type. B, immunoblot (IB) showing equivalent loading of DN-Cul3 and DN-Cul3mut in each glioblastoma cell line used for soft-growth agar assays and xenograft injections shown in A and C. Vinculin levels are shown as loading control. C, dot plot depicting differences in tumor volume in xenograft tumor-bearing mice injected with U87 or LN319 cells expressing DN-Cul3 or a (L52AE55A) DN-Cul3mut control (same cells as in A). P value significance in the panel was determined by Student t test. n = 6 each. n.s., not statistically significant. D, relative tumor incidence of U87-shp53 glioblastoma cells expressing DN-Cul3 or a control DN-Cul3mut (L52AE55A) vector in conjunction with a lentiviral NF1 shRNA or a control shRNA. Bars represent the fraction of tumor incidence over a period of 5 months and the number of tumors formed/number of injections are depicted. n = 8 in each category. P = 0.0014 (DN-Cul3;shScr versus DN-Cul3;shNF1) using Fisher exact test. E, dot plot depicting tumor volume in xenograft-tumor bearing mice injected with U87-shp53 glioblastoma cells expressing DN-Cul3 or the control DN-Cul3mut (L52AE55A) vector together with a lentiviral NF1 shRNA or a control shRNA. A Mann–Whitney U test (P = 0.0025) was used to illustrate a significant difference in tumor volume in xenograft tumors composed of DN-Cul3;shScr versus those comprising DN-Cul3;shNF1-expressing cells. F, neurofibromin expression in shp53-U87 cells used for xenograft studies shown in D and E. Cells express shNF1 or a control shRNA and HA-tagged DN-Cul3 or DN-Cul3mut constructs. HA immunoblot showing equivalent expression of DN-Cul3 and DN-Cul3mut is shown, as well as RasGAPp120 which serves as a loading control. Pablo E. Hollstein, and Karen Cichowski Cancer Discovery 2013;3:880-893 ©2013 by American Association for Cancer Research