Volume 158, Issue 6, Pages (September 2014)

Slides:



Advertisements
Similar presentations
Robert L. Unckless, Virginia M. Howick, Brian P. Lazzaro 
Advertisements

Hair Keratin Associated Proteins: Characterization of a Second High Sulfur KAP Gene Domain on Human Chromosome 211  Michael A. Rogers, Hermelita Winter,
Volume 14, Issue 1, Pages (July 2013)
John F. Golz, Emma J. Keck, Andrew Hudson  Current Biology 
Hair Keratin Associated Proteins: Characterization of a Second High Sulfur KAP Gene Domain on Human Chromosome 211  Michael A. Rogers, Hermelita Winter,
Colponemids Represent Multiple Ancient Alveolate Lineages
Jianbin Wang, H. Christina Fan, Barry Behr, Stephen R. Quake  Cell 
Volume 8, Issue 2, Pages (February 2015)
A Family of Zinc-Finger Proteins Is Required for Chromosome-Specific Pairing and Synapsis during Meiosis in C. elegans  Carolyn M. Phillips, Abby F. Dernburg 
Mapping Human Epigenomes
Volume 19, Issue 5, Pages (May 2011)
Roger B. Deal, Steven Henikoff  Developmental Cell 
The Evolution of Venom by Co-option of Single-Copy Genes
Volume 124, Issue 1, Pages (January 2006)
Volume 22, Issue 6, Pages (June 2014)
Evolutionary Inference across Eukaryotes Identifies Specific Pressures Favoring Mitochondrial Gene Retention  Iain G. Johnston, Ben P. Williams  Cell.
Volume 91, Issue 3, Pages (August 2016)
Impulse Control: Temporal Dynamics in Gene Transcription
Michael J. Metzger, Carol Reinisch, James Sherry, Stephen P. Goff  Cell 
A Massively Parallel Reporter Assay of 3′ UTR Sequences Identifies In Vivo Rules for mRNA Degradation  Michal Rabani, Lindsey Pieper, Guo-Liang Chew,
Volume 20, Issue 12, Pages (June 2010)
Luisa De Sordi, Varun Khanna, Laurent Debarbieux  Cell Host & Microbe 
CA3 Retrieves Coherent Representations from Degraded Input: Direct Evidence for CA3 Pattern Completion and Dentate Gyrus Pattern Separation  Joshua P.
Volume 117, Issue 3, Pages (September 1999)
Volume 154, Issue 1, Pages (July 2013)
Volume 138, Issue 5, Pages (September 2009)
John F. Golz, Emma J. Keck, Andrew Hudson  Current Biology 
A Novel Family of Candidate Pheromone Receptors in Mammals
Ribosomal Protein L3: Gatekeeper to the A Site
Volume 18, Issue 5, Pages (November 2015)
Volume 15, Issue 1, Pages (January 2007)
James G. Heys, Krsna V. Rangarajan, Daniel A. Dombeck  Neuron 
The Human Condition—A Molecular Approach
Volume 125, Issue 7, Pages (June 2006)
The Role of the RNAi Machinery in Heterochromatin Formation
Volume 137, Issue 3, Pages (May 2009)
RNA Structural Determinants of Optimal Codons Revealed by MAGE-Seq
EB3 Regulates Microtubule Dynamics at the Cell Cortex and Is Required for Myoblast Elongation and Fusion  Anne Straube, Andreas Merdes  Current Biology 
The Ribosome Emerges from a Black Box
Volume 63, Issue 4, Pages (August 2016)
Volume 124, Issue 5, Pages (March 2006)
Volume 22, Issue 15, Pages (August 2012)
Sex Chromosome Specialization and Degeneration in Mammals
Structural Basis for Endosomal Targeting by the Bro1 Domain
Volume 20, Issue 4, Pages (November 2005)
Gautam Dey, Tobias Meyer  Cell Systems 
A DNA Replication Mechanism for Generating Nonrecurrent Rearrangements Associated with Genomic Disorders  Jennifer A. Lee, Claudia M.B. Carvalho, James.
Michael A. Rogers, Hermelita Winter, Christian Wolf, Jürgen Schweizer 
Volume 122, Issue 6, Pages (September 2005)
Cetaceans on a Molecular Fast Track to Ultrasonic Hearing
Identical Skin Toxins by Convergent Molecular Adaptation in Frogs
James H. Marshel, Takuma Mori, Kristina J. Nielsen, Edward M. Callaway 
Structure of the BRCT Repeats of BRCA1 Bound to a BACH1 Phosphopeptide
Volume 14, Issue 4, Pages (October 2013)
Maximum likelihood phylogeny of USA500 and other CC8 strains.
STIL Microcephaly Mutations Interfere with APC/C-Mediated Degradation and Cause Centriole Amplification  Christian Arquint, Erich A. Nigg  Current Biology 
Bálint Lasztóczi, Thomas Klausberger  Neuron 
Patterns of Stem Cell Divisions Contribute to Plant Longevity
Matthew A. Campbell, Piotr Łukasik, Chris Simon, John P. McCutcheon 
Colponemids Represent Multiple Ancient Alveolate Lineages
Volume 22, Issue 6, Pages (June 2014)
Volume 25, Issue 8, Pages e3 (August 2017)
Volume 153, Issue 7, Pages (June 2013)
Horizontal gene transfer and the evolution of cnidarian stinging cells
Fig. 1. —OR gene tree including 2,973 genes from seven ants, honeybee, and jewel wasp. The tree was reconstructed ... Fig. 1. —OR gene tree including 2,973.
Volume 21, Issue 23, Pages (December 2011)
The Structure of T. aquaticus DNA Polymerase III Is Distinct from Eukaryotic Replicative DNA Polymerases  Scott Bailey, Richard A. Wing, Thomas A. Steitz 
CRISPR Immunological Memory Requires a Host Factor for Specificity
1 2 Biology Warm Up Day 6 Turn phones in the baskets
Robert L. Unckless, Virginia M. Howick, Brian P. Lazzaro 
Presentation transcript:

Volume 158, Issue 6, Pages 1270-1280 (September 2014) Sympatric Speciation in a Bacterial Endosymbiont Results in Two Genomes with the Functionality of One  James T. Van Leuven, Russell C. Meister, Chris Simon, John P. McCutcheon  Cell  Volume 158, Issue 6, Pages 1270-1280 (September 2014) DOI: 10.1016/j.cell.2014.07.047 Copyright © 2014 Elsevier Inc. Terms and Conditions

Cell 2014 158, 1270-1280DOI: (10.1016/j.cell.2014.07.047) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 1 Origin of Duplicated Hodgkinia Genomes in the Cicada Genus Tettigades At left, an unrooted maximum likelihood phylogeny based on cicada COI is shown with bootstrap support values (the scale bar is 0.1 expected substitutions per site; MAGTRE is Magicicada tredecim). The number of Hodgkinia genomes are indicated by colored circles to the right of the tree. The ancestral nature of the single Hodgkinia genome is evident from the sister group relationship between TETULN and the clade containing TETUND and TETAUR. The right side of the figure shows representative sections of genome, where intact genes are shown by large colored boxes, gene loss is indicated by empty boxes, and pseudogenes are shown as small open reading frames broken by frameshifts (small filled boxes) and stop codons (asterisks). Cell 2014 158, 1270-1280DOI: (10.1016/j.cell.2014.07.047) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 2 Reciprocal Patterns of Gene Loss and Retention in TETUND1 and TETUND2 (A) Venn diagram showing genes retained in the TETULN and TETUND genomes. (B) Nutrient-provisioning genes encoded on the TETULN and DICSEM (green circles), TETUND1 (orange circles), TETUND2 (blue circles) genomes or missing or pseudogenized (open circles). (C) Patterns of TETUND gene retention, pseudogene formation, and rates of amino acid evolution mapped onto the TETULN genome. Annotated genes on the TETULN genome are shown as green boxes along the center of the image. Gray boxes are RNA genes. White boxes are genes that have been deleted in either TETUND1 or TETUND2 or both. If a gene is present and apparently functional on TETUND1, it is shown as a dark-orange box, the height of which is proportional to the number of amino acid substitutions between the TETUND1 protein and the homolog in TETULN. If a gene is present as a pseudogene on TETUND1, it is shown as a light-orange box. TETUND2 genes follow the same pattern as TETUND1 but are shown as blue bars below the TETULN genome. Rates of 0.5 and 1.0 amino acid changes per site are shown as horizontal black lines. Figure 1 details the genomic region highlighted in light gray (the first eight genes in the genome). See also Table S1. Cell 2014 158, 1270-1280DOI: (10.1016/j.cell.2014.07.047) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 3 Differential Rates of Amino Acid Sequence Evolution Identify Possible Incipient Psuedogenes Homologs present in TETULN, TETUND1, and TETUND2 are shown as gray dots. Homologs pseudogenized in TETUND1 or TETUND2 are shown as orange and blue dots, respectively. Genes lost in either TETUND1 or TETUND2 are shown as purple dots along the axis. The five putative incipient pseudogenes (rplU, rpsK, rplP, rpmJ, and hisB) are shown as black dots. The graph is cropped at one expected amino acid change per site. See also Table S2. Cell 2014 158, 1270-1280DOI: (10.1016/j.cell.2014.07.047) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 4 FISH Microscopy (A) rRNA-targeted probes distinguish Sulcia (green) from Hodgkinia (red). (B) Genome-targeted probes distinguish Sulcia (green), TETUND1 (yellow), and TETUND2 (blue). Hoechst-stained DNA is colored magenta and primarily stains insect nuclei. Scale bar, 20 μm. See also Figure S1 and Movie S1. Cell 2014 158, 1270-1280DOI: (10.1016/j.cell.2014.07.047) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 5 A Model for the Splitting of the Ancestral Hodgkinia Lineage (A) We assume that Hodgkinia started as a population of cells with a single polyploid ancestral genotype (shown as green circles). (B) Mutations that inactivate at least one gene occur in two different Hodgkinia cells (orange and blue boxes) in the same insect. (C) These inactivating mutations rise to high levels, masked by the polyploid nature of Hodgkinia. (D) A bottleneck event purges the ancestral Hodgkinia genotype and fixes the reciprocal inactivating mutations in the population. (E) These new species lose genes in a reciprocal fashion to give rise to two discrete Hodgkinia genomes (orange and blue circles). Cell 2014 158, 1270-1280DOI: (10.1016/j.cell.2014.07.047) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure S1 FISH Microscopy Using Genome-Targeted Probes Distinguish Hodgkinia in Tettigades near undata, Related to Figure 4 (A) The two Hodgkinia genomes (blue and yellow) show from bacteriome tissue thin-sections as described in Figure 4. Hoechst stained DNA is colored magenta, and primarily stains insect nuclei; no Sulcia probe was used in this experiment. (B) DIC image shows characteristic cell morphology, with Hodgkinia cells surrounded by Sulcia cells. Scale bar is 50 μm. Cell 2014 158, 1270-1280DOI: (10.1016/j.cell.2014.07.047) Copyright © 2014 Elsevier Inc. Terms and Conditions