Influence of MS measurement conditions on the deviation factors between the estimated and measured concentrations of 46 proteins in neuro2a cells.A, QSTAR with faster scan cycle (0.55 s for each MS/MS scan). Influence of MS measurement conditions on the deviation factors between the estimated and measured concentrations of 46 proteins in neuro2a cells.A, QSTAR with faster scan cycle (0.55 s for each MS/MS scan). B, QSTAR with slower scan cycle (1.5 s for each MS/MS scan). C, LCQ with slower scan cycle (1.2 s on average for each MS/MS scan). D, LTQ with faster scan cycle (0.38 s on average for each MS/MS scan). E, multidimensional LC-MS/MS with LTQ (five fractions). F, multidimensional LC-MS/MS with QSTAR and LTQ. Yasushi Ishihama et al. Mol Cell Proteomics 2005;4:1265-1272 The American Society for Biochemistry and Molecular Biology