Corticoids synergize with IL-1 in the induction of LCN2

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Corticoids synergize with IL-1 in the induction of LCN2 J. Conde, V. Lazzaro, M. Scotece, V. Abella, R. Villar, V. López, M.Á. Gonzalez-Gay, J. Pino, R. Gómez, A. Mera, O. Gualillo Osteoarthritis and Cartilage Volume 25, Issue 7, Pages 1172-1178 (July 2017) DOI: 10.1016/j.joca.2017.01.017 Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 mLCN2 mRNA expression determined by RT-qPCR. ∗indicates P < 0.05; ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs unstimulated control. Osteoarthritis and Cartilage 2017 25, 1172-1178DOI: (10.1016/j.joca.2017.01.017) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 mLCN2 protein expression determined by western blot analysis. A, B low panels. Data showing densitometric analysis of all performed western blots. C, D. mMMP-13 expression determined by RT-qPCR. ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs unstimulated control. #indicates P < 0.05; ##indicates P < 0.01 and ###indicates P < 0.001 vs IL-1α (0.1 ng/mL). Osteoarthritis and Cartilage 2017 25, 1172-1178DOI: (10.1016/j.joca.2017.01.017) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 mLCN2 protein expression determined by western blot analysis. A, B low panels. Data showing densitometric analysis of all performed western blots. ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs unstimulated control. ##indicates P < 0.01 and ###indicates P < 0.001 vs PRED or ALD (1 μM) plus IL-1α (0.1 ng/mL). Osteoarthritis and Cartilage 2017 25, 1172-1178DOI: (10.1016/j.joca.2017.01.017) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 A, B mLCN2 protein expression determined by western blot analysis. A, B low panels. Data showing densitometric analysis of all performed western blots. ∗indicates P < 0.05; ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs unstimulated control. ##indicates P < 0.01 and ###indicates P < 0.001 vs PRED or ALD (1 μM) plus IL-1α (0.1 ng/mL). C, D, E. mLCN2 mRNA expression determined by RT-qPCR. ∗indicates P < 0.05; ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs IL-1α, PRED or ALD. Osteoarthritis and Cartilage 2017 25, 1172-1178DOI: (10.1016/j.joca.2017.01.017) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions