Neutropenia impairs venous thrombosis resolution in the rat

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Presentation transcript:

Neutropenia impairs venous thrombosis resolution in the rat Manu R Varma, BS, Andrea J Varga, BS, Brian S Knipp, MS, Pasu Sukheepod, MD, Gilbert R Upchurch, MD, Steven L Kunkel, PhD, Thomas W Wakefield, MD, Peter K Henke, MD Journal of Vascular Surgery Volume 38, Issue 5, Pages 1090-1098 (November 2003) DOI: 10.1016/S0741-5214(03)00431-2

Fig 1 Study design. This chart details the sequence of rabbit anti-rat PMN serum (anti-PMN serum) or control rabbit serum injection, IVC ligation, sacrifice, and the outcome measurements assessed in this study. Journal of Vascular Surgery 2003 38, 1090-1098DOI: (10.1016/S0741-5214(03)00431-2)

Fig 2 The inflammatory response within the thrombus was assessed by immunohistochemical staining for (A) PMNs and (B) monocytes, as well as the (C) MPO assay. (A) There were significantly fewer intrathrombus PMNs in neutropenic rats at 2 and 4 days (both P < .05), but no difference at 7 days. (B) There were no differences in intrathrombus monocytes among any groups. (C) There was significantly less MPO activity among 4-day neutropenic rats compared with 4-day controls, but no differences were detectable at other time points. Journal of Vascular Surgery 2003 38, 1090-1098DOI: (10.1016/S0741-5214(03)00431-2)

Fig 3 Representative immunohistochemical staining of thrombi from (A) control and (B) neutropenic rats (200×). There are far fewer PMNs visible within the thrombi of neutropenic rats. Journal of Vascular Surgery 2003 38, 1090-1098DOI: (10.1016/S0741-5214(03)00431-2)

Fig 4 Thrombus size. Neutropenic rats had thrombi with larger weight/length ratios at 2 days and 7 days (P < .05), but there was no difference in thrombus size at 4 days. Journal of Vascular Surgery 2003 38, 1090-1098DOI: (10.1016/S0741-5214(03)00431-2)

Fig 5 Venograms from (A) control and (B) neutropenic rats taken at 7-days post-IVC ligation. (A) The thrombus of the control rats had greater recanalization, and less IVC thrombus area, which was not seen in the neutropenic rats (B), where larger IVC thrombus area and more extensive collateral formation was seen. Journal of Vascular Surgery 2003 38, 1090-1098DOI: (10.1016/S0741-5214(03)00431-2)

Fig 6 A, Sircol collagen assay. There was significantly more collagen (types I to III) remaining in the thrombi of neutropenic rats compared with controls at 7 days. This is reflected in Trichrome-stained section (100×) from (B) control and (C) neutropenic rat thrombi. Note increased fibrosis within the thrombus proper as well as the vein wall in the neutropenic IVC section, stained whitish-blue. Clot is red color. T = thrombus. W = vein wall. Journal of Vascular Surgery 2003 38, 1090-1098DOI: (10.1016/S0741-5214(03)00431-2)