Experiment Questions Enzyme pH.

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Experiment Questions Enzyme pH

Enzyme pH Name the enzyme and substrate that you used when you carried out an experiment to investigate the effect of pH on the rate of enzyme action. Enzyme + Substrate Catalase and H2O2 Amylase and Starch

Explain how you varied the pH? Used different Buffers Enzyme pH Explain how you varied the pH? Used different Buffers

Enzyme pH State one factor that you kept constant. Temperature/enzyme conc./substrate conc.

The pH at which enzymes works best is known as the … pH. Optimum Enzyme pH The pH at which enzymes works best is known as the … pH. Optimum

Enzyme pH Explain what is meant by the term pH. (A measure of) how acidic or alkaline a solution

Enzyme pH For what purpose did you use buffer solutions in the course of your practical activities? To control the pH

Enzyme pH Name (i) The enzyme you used in this investigation. (ii) The substrate of this enzyme. (iii) The product of this enzyme. (i) Amylase or Pepsin or Catalase (ii) Matching substrate (iii) Matching product

How did you vary the pH? Add acid or add base or add different buffers Enzyme pH How did you vary the pH? Add acid or add base or add different buffers

Enzyme pH (i) Name one factor you kept constant. (ii) How did you keep that factor constant? (i) Temperature (ii) Water bath