Detection and Quantification of BCR-ABL1 Fusion Transcripts by Droplet Digital PCR Lawrence J. Jennings, David George, Juliann Czech, Min Yu, Loren Joseph

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Detection and Quantification of BCR-ABL1 Fusion Transcripts by Droplet Digital PCR Lawrence J. Jennings, David George, Juliann Czech, Min Yu, Loren Joseph The Journal of Molecular Diagnostics Volume 16, Issue 2, Pages 174-179 (March 2014) DOI: 10.1016/j.jmoldx.2013.10.007 Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Log-log plot demonstrating correlation of BCR-ABL1/BCR ratios to IS calibrators. Total RNA was extracted from pooled patient samples and diluted in total RNA from healthy donors. Replicates were tested together with IS calibrators by two technologists over 20 days. Data show near perfect correlation between sample dilutions and calibrators. The Journal of Molecular Diagnostics 2014 16, 174-179DOI: (10.1016/j.jmoldx.2013.10.007) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Log transformations of BCR-ABL1/BCR ratios show linearity and lack of bias. For both the dilution samples and the IS calibrator samples, the slope equals 1, indicating no concentration-dependent bias, and R2 is nearly 1, indicating remarkable correlation. The Journal of Molecular Diagnostics 2014 16, 174-179DOI: (10.1016/j.jmoldx.2013.10.007) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions