Volume 70, Issue 1, Pages 170-176 (July 2006) Effect of spironolactone and captopril on nitric oxide and S-nitrosothiol formation in kidney of L-NAME-treated rats  O. Pechanova, J. Matuskova, D. Capikova, L. Jendekova, L. Paulis, F. Simko  Kidney International  Volume 70, Issue 1, Pages 170-176 (July 2006) DOI: 10.1038/sj.ki.5001513 Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 1 Effect of 4-week L-NAME, spironolactone, L-NAME+spironolactone, captopril, and L-NAME+captopril treatment on SBP. Ctrl – control, LN – 40 mg/kg/day L-NAME, S – spironolactone 200 mg/kg/day, SL – L-NAME+spironolactone in corresponding doses, C – captopril 100 mg/kg/day, CL – L-NAME+captopril in corresponding doses. Significant differences: *P<0.05 compared to controls, +P<0.05 compared to L-NAME group. Kidney International 2006 70, 170-176DOI: (10.1038/sj.ki.5001513) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 2 Effect of 4-week L-NAME, spironolactone, L-NAME+spironolactone, captopril, and L-NAME+captopril treatment on NOS activity in the kidney. Ctrl – control, LN – 40 mg/kg/day L-NAME, S – spironolactone 200 mg/kg/day, LS – L-NAME+spironolactone in corresponding doses, C – captopril 100 mg/kg/day, LC – L-NAME+captopril in corresponding doses. *P<0.05 compared to control, +P<0.05 compared to L-NAME group. Kidney International 2006 70, 170-176DOI: (10.1038/sj.ki.5001513) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 3 Effect of 4-week L-NAME+ spironolactone, L-NAME+spironolactone, captopril, and L-NAME+captopril treatment on eNOS and β-actin expression in the kidney. Ctrl – control, LN – 40 mg/kg/day L-NAME, S – spironolactone 200 mg/kg/day, LS – L-NAME+spironolactone in corresponding doses, C – captopril 100 mg/kg/day, LC – L-NAME+captopril in corresponding doses. *P<0.05 compared to control, +P<0.05 compared to L-NAME group. Kidney International 2006 70, 170-176DOI: (10.1038/sj.ki.5001513) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 4 Effect of 4-week L-NAME, spironolactone, L-NAME+spironolactone, captopril, and L-NAME+captopril treatment on iNOS and β-actin expression in the kidney. Ctrl – control, LN – 40 mg/kg/day L-NAME, S – spironolactone 200 mg/kg/day, LS – L-NAME+spironolactone in corresponding doses, C – captopril 100 mg/kg/day, LC – L-NAME+captopril in corresponding doses. Kidney International 2006 70, 170-176DOI: (10.1038/sj.ki.5001513) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 5 Effect of 4-week L-NAME, spironolactone, L-NAME+spironolactone, captopril, and L-NAME+captopril treatment on thiol group concentration in the kidney. Ctrl – control, LN – 40 mg/kg/day L-NAME, S – spironolactone 200 mg/kg/day, LS – L-NAME+spironolactone in corresponding doses, C – captopril 100 mg/kg/day, LC – L-NAME+captopril in corresponding doses. *P<0.05 compared to control, +P<0.05 compared to L-NAME group. Kidney International 2006 70, 170-176DOI: (10.1038/sj.ki.5001513) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 6 Effect of 4-week L-NAME, spironolactone, L-NAME+spironolactone, captopril, and L-NAME+captopril treatment on S-nitrosothiol group concentration in the kidney. Ctrl – control, LN – 40 mg/kg/day L-NAME, S – spironolactone 200 mg/kg/day, LS – L-NAME+spironolactone in corresponding doses, C – captopril 100 mg/kg/day, LC – L-NAME+captopril in corresponding doses. *P<0.05 compared to control, +P<0.05 compared to L-NAME group. Kidney International 2006 70, 170-176DOI: (10.1038/sj.ki.5001513) Copyright © 2006 International Society of Nephrology Terms and Conditions