Reflections on a Novel Therapeutic Candidate

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Reflections on a Novel Therapeutic Candidate Letha J Sooter, Andrew D Ellington Chemistry & Biology Volume 9, Issue 8, Pages 857-858 (August 2002) DOI: 10.1016/S1074-5521(02)00190-4

Figure 1 In Vitro Selection Process for DNA Aptamers and Spiegelmers (1), Single-stranded, randomized D-DNA pool containing approximately 1015 different species; (2), DNA pool is incubated with target molecules and allowed to bind; (3), nonbinding species are partitioned away from binding species; (4), binding species are PCR amplified; (5), PCR product is single-strand separated, and the leading strand is subjected to further rounds of selection; (6), after several rounds of selection, binding species are cloned, sequenced, and assayed for activity. Successful binders are resynthesized using L-nucleotides. Chemistry & Biology 2002 9, 857-858DOI: (10.1016/S1074-5521(02)00190-4)