Indirect immunofluorescence analysis of 14-3-3σ in bladder specimens, in normal urothelium (A, cyst 981; B, cyst 981; C, cyst 1004; and D, cyst 975), and invasive TCCs (E, 898-3 pTa G1; F, 738; G, 702 pT2-4 G3; H, 697 pT1 G3; I, 566 pT2-4 G4). Indirect immunofluorescence analysis of 14-3-3σ in bladder specimens, in normal urothelium (A, cyst 981; B, cyst 981; C, cyst 1004; and D, cyst 975), and invasive TCCs (E, 898-3 pTa G1; F, 738; G, 702 pT2-4 G3; H, 697 pT1 G3; I, 566 pT2-4 G4). Original magnification, ×100 (A, D–I) and ×400 (B and C). Grading was performed by an experienced pathologist and was based on hematoxylin and eosin-stained sections. José M. A. Moreira et al. Mol Cell Proteomics 2004;3:410-419 © 2004 The American Society for Biochemistry and Molecular Biology