Fig. 1 Differentiation of human peripheral blood monocytes into MDMi cells induces a microglial gene expression and functional phenotype. Differentiation.

Slides:

Advertisements

Similar presentations

Volume 8, Issue 6, Pages (June 2017)

Advertisements

Figure 4. Inhibition of LPS-induced IL-1β and TNFα, but not IL-6, after exercise. Depicted are LPS-induced IL-1β (upper panel), IL-6 (middle panel), and.

Fig. 8. Recurrent copy number amplification of BRD4 gene was observed across common cancers. Recurrent copy number amplification of BRD4 gene was observed.

Volume 8, Issue 6, Pages (June 2017)

Fig. 4. PVSRIPO infection of DCs is sublethal, is marginally productive, and induces sustained proinflammatory cytokine production. PVSRIPO infection of.

Ex vivo effects of proNGF and NGF on JIA mononuclear cells.

Fig. 3. Cytokines trigger proliferation and block differentiation of perivascular adipocytes. Cytokines trigger proliferation and block differentiation.

Transfer of miR-223 during neutrophil-epithelial cell interactions

Fig. 5. Circulating PPi concentration does not correlate with severity of calcification phenotype in mice. Circulating PPi concentration does not correlate.

In vivo function of MeTro sealants using rat incision model of lungs

Fig. 4. Bexarotene promotes PPARδ activation of target genes in mouse brain and muscle. Bexarotene promotes PPARδ activation of target genes in mouse brain.

IRF4 regulates cytokine receptor expression in CD8+ T cells.

Fig. 8. Gene and protein changes in ALK-dependent STING pathways in human sepsis. Gene and protein changes in ALK-dependent STING pathways in human sepsis.

Fig. 1. APP/PS1;C3 KO mice show improved cognitive flexibility (reversal) compared to APP/PS1 mice at 16 months of age. APP/PS1;C3 KO mice show improved.

Fig. 4. Ex vivo and in vivo function of the MeTro sealant using rat incision model of arteries. Ex vivo and in vivo function of the MeTro sealant using.

Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.

Fig. 2. Engraftment of CART-EGFRvIII and cytokine modulation in the peripheral blood. Engraftment of CART-EGFRvIII and cytokine modulation in the peripheral.

Fig. 1 SDPS induces deficits in rat spatial memory that are reversed by imipramine. SDPS induces deficits in rat spatial memory that are reversed by imipramine.

Fig. 5. Wisper is associated with TIA1-related protein and regulates lysyl hydroxylase 2 expression. Wisper is associated with TIA1-related protein and.

Analysis of brain and spinal cord of treated Gaa−/− mice and controls

PVSRIPO-mediated APC activation occurs in immunosuppressive conditions

Fig. 1. Identification of SE-associated lncRNAs.

Fig. 1 ZIKV-related flaviviruses cause fetal demise in mice that can be prevented by mAb treatment. ZIKV-related flaviviruses cause fetal demise in mice.

Fig. 5. Antitumor efficacy of ERY974 in immunocompetent human CD3 transgenic mice. Antitumor efficacy of ERY974 in immunocompetent human CD3 transgenic.

Fig. 7 Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors. Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors.

by Melissa R. Miller, Nadja Mannowetz, Anthony T

Fig. 2. Vascular inflammation blocks perivascular adipocyte differentiation through paracrine signals. Vascular inflammation blocks perivascular adipocyte.

Fig. 5 Hypoxic tumors from obese mice associate with increased production of IL-6 by adipocytes and myeloid cells. Hypoxic tumors from obese mice associate.

Fig. 4. Expression of HGF in liver ECs cooperates with NOX4 inhibition to enhance engraftment of regenerative hepatocytes. Expression of HGF in liver ECs.

Transcriptional Profiling of Quiescent Muscle Stem Cells In Vivo

Persistence of CAR4 cells is reduced after sustained TCR engagement

Fibronectin is a TH1-specific molecule in human subjects

Fig. 7. KIF11 informs patient prognosis, and targeting improves survival in a preclinical model. KIF11 informs patient prognosis, and targeting improves.

Fig. 1. mGlu7 expression is reduced in RTT autopsy samples.

Fig. 5 Local gel scaffold for T cell memory response.

Fig. 3. ERRα is involved in the regulation of OPLAH.

Fig. 1. PGBD5-expressing cells do not tolerate deficiency of nonhomologous end-joining DNA repair. PGBD5-expressing cells do not tolerate deficiency of.

Fibronectin is a TH1-specific molecule in human subjects

Stroke induces atheroprogression via the RAGE-signaling pathway

Fig. 3. Morphological changes associated with glial activation were reduced in 16-month-old APP/PS1;C3 KO mice. Morphological changes associated with glial.

Fig. 2. IL-2/rapamycin–expanded T cells express homing receptors to traffic to lymphoma sites and are resistant to SN-38 toxicity. IL-2/rapamycin–expanded.

PAAND is driven by local inflammasome activation and IL-1β production

Fig. 2 Genotype-induced differential gene expression is different in MDMi cells compared to monocytes. Genotype-induced differential gene expression is.

Fig. 1. Bexarotene promotes PPARδ activation to ameliorate the neurotoxicity of mutant huntingtin. Bexarotene promotes PPARδ activation to ameliorate the.

Fig. 1. Generation of forebrain progenitor cells with regional identity from human iPSCs. Generation of forebrain progenitor cells with regional identity.

Fig. 1. In vivo fates of patient-derived AML cells defined by mutational profile. In vivo fates of patient-derived AML cells defined by mutational profile.

Fig. 1 CSPG4 is expressed in GBM specimens and GBM-NS and associated with more aggressive disease. CSPG4 is expressed in GBM specimens and GBM-NS and associated.

Bexarotene is neuroprotective in mouse and human HD neurons in vitro

Fig. 3 CSF1 is expressed in human melanoma.

Fig. 1 Characterization of hESC-RPE cells.

Stroke induces inflammatory activation of the aortic endothelium

Fig. 2 Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART results in successive increases in elongated, polyadenylated, and.

Fig. 3 Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART selectively reverses baseline blocks to splicing, elongation, and.

CSF1 secretion by melanoma cells is induced by CTL-derived cytokines

Fig. 4 ALRN-6924 inhibits cellular proliferation and clonogenic capacity, and induces cell cycle arrest and apoptosis in AML cell lines. ALRN-6924 inhibits.

Fig. 2. BET inhibition enhances PARPi-induced DNA damage.

Relative expression levels of known mRNA selected as points of reference. Relative expression levels of known mRNA selected as points of reference. mRNA.

Fig. 4. Induced Zbtb7a in lungs is localized to AMs.

Adenosine-induced osteogenic differentiation of hiPSCs involves A2bR

Effect of YopM on the transcription of pro-inflammatory cytokines in HL60-derived macrophages after stimulation with LPS. HL60 cells differentiated into.

hiPSC-CMs exhibit sarcomeric proteins and express human RTK families

High maternal expression of Cnot6l, an active component of the CCR4–NOT deadenylase complex. High maternal expression of Cnot6l, an active component of.

Fig. 1. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy.

CD49b+ cells from tumor-bearing mice are more prone to conversion into MDSCs compared with naive CD49b+ cells. CD49b+ cells from tumor-bearing mice are.

Fig. 1 A time-course RNA-seq analysis reveals prominent IRF8 expression in the spinal cord during the recovery phase after SCI. A time-course RNA-seq analysis.

Targeted nanoparticles containing the proresolving peptide Ac2-26 protect against advanced atherosclerosis in hypercholesterolemic mice by Gabrielle Fredman,

Fig. 2. Col IV–Ac2-26 NPs increase subendothelial collagen in Ldlr−/− mice with established atherosclerosis. Col IV–Ac2-26 NPs increase subendothelial.

Fig. 3. Col IV–Ac2-26 NPs suppress lesional superoxide and increase lesional Il10 mRNA in Ldlr−/− mice with established atherosclerosis. Col IV–Ac2-26.

Fig. 1 Differentiation of human peripheral blood monocytes into MDMi cells induces a microglial gene expression and functional phenotype. Differentiation.

Fig. 4 Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. Rotavirus induces the type I IFN pathway and release of ATP by tumor.

Presentation transcript:

Fig. 1 Differentiation of human peripheral blood monocytes into MDMi cells induces a microglial gene expression and functional phenotype. Differentiation of human peripheral blood monocytes into MDMi cells induces a microglial gene expression and functional phenotype. Human peripheral blood monocytes from young, healthy subjects were incubated with cytokines and differentiated into monocyte-derived microglia-like (MDMi) cells. (A) The cell type–specific enriched gene expression for MDMi cells, ex vivo human microglia (HuMG), ex vivo murine microglia (P60MG), and human embryonic and induced pluripotent stem cell–derived microglia (ESC/iPSC)–derived microglia (pMGL) were compared. (B) Four genes defined as being microglia-specific in mice were significantly up-regulated in MDMi cells (TGFβR1: ****P < 0.0001; PROS1: ***P = 0.0005; C1QB: ***P = 0.0007; P2RX7: **P = 0.0058) at day 10 of differentiation compared to freshly isolated monocytes and MDM cells from the same five individuals. Gene expression was quantified using RNA sequencing and expressed as fragments per kilobase of transcript per million (FPKM). One-way analysis of variance (ANOVA) with Tukey’s post hoc test. (C) P2RY12 and TMEM119 proteins were more highly expressed in MDMi cells compared to monocytes. (D) MDMi cells functionally mimicked human microglia in response to conditions that led to either an M1 or M2 phenotype. Under M1 conditions, MDMi cells expressed significantly more interleukin-10 (IL-10) mRNA (**P < 0.01) compared to MDM cells from the same individuals. Student’s t test, n = 14. For (B) and (D), each dot represents a biological replicate. Horizontal line denotes the mean. DAPI, 4′,6-diamidino-2-phenylindole. Katie J. Ryan et al., Sci Transl Med 2017;9:eaai7635 Published by AAAS