Suicide Ideation in the depression group (NBB)

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Presentation transcript:

Suicide Ideation in the depression group (NBB) A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting to detailed planning, role playing, self-harm and attempts Anterior Cingulate Cortex * * ** DLPFC was also checked, however, none of the target genes were unchanged. Juan Zhao and Dick Swaab, submitted

Microglia isolation procedure Mechanic & enzymatic dissociation Cell separation: Percoll gradient Take off myelin Collect cells from interface Stain cells with labeled antibodies: CD11b - PE CD45 - FITC CD15 - APC Selection using magnetic CD11b beads Melief et al. Glia 2012

Isolation and phenotyping of pure primary human microglia

Peripheral inflammation impacts on microglia activation Melief et al. Glia 2012

The Netherlands Brain Bank for Psychiatry

Phenotype & Characterize human post mortem microglia Jeroen Melief, thesis 2014