Day Three

OBJECTIVES Learn to analyze the results from DNA electrophoresis Identify and investigate problems scientifically Communicate scientific investigations and information clearly.

Housekeeping You will turn in your Lab checklist (peer-graded) TODAY! We will finish up the lab and finish the post lab write-up Genetics Re-takes can be done today during lunch Study for post-lab test

Warm-up 1. What was the purpose of loading the DNA size standard in the first well? 2. What was the purpose of adding the loading dye (LD) to each sample? 3. Reflect on the previous labs. What went well? What did not go well? Any thing you can improve on?

Analysis of Stained Gel Determine the size of DNA fragments Identify the related samples

Who’s bands match up the blood stain band?

Is he the child’s father?

Let’s analyze your gel!

Let’s Think About It!!! What do the bands represent? How do the bands on the bottom of the gel compare to those at the top? What would happen if you did not add the restriction enzyme? Sketch it What would it look like if you did not have any DNA in your sample? Sketch What would it look like if you used the same tip for all of your DNA samples?

ST CS S1 S2 S3 S4 S5 ST CS S1 S2 S3 S4 S5 THIS IS FROM THE WEBSITE!