Comparison of macrorestriction analysis of genomic DNA by pulsed-field gel electrophoresis and ribotyping with conventional methods for differentiation.

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Presentation transcript:

Comparison of macrorestriction analysis of genomic DNA by pulsed-field gel electrophoresis and ribotyping with conventional methods for differentiation of Escherichia coli 0124 isolates Teresa Llovet, Pere Coll, Francesca March, Isaí Montserrat, Isabel Atela, Beatriz Mirelis, Guillermo Prats Clinical Microbiology and Infection Volume 1, Issue 2, Pages 127-133 (December 1995) DOI: 10.1111/j.1469-0691.1995.tb00457.x Copyright © 1995 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Ribotypes generated by BglII for strains of EIEC 0124. Lane 1 to 7, sporadic isolates (lane 1, strain 25; lane 2, strain 26; lane 3, strain 106; lane 4, strain 139; lane 5, strain 140; lane 6, strain 141; lane 7, strain 142); lane 8, outbreak I; lane 9, outbreak II; lane Mk, size marker (Xenorhabdus 278 chromosomal DNA digested with EcoRI). Clinical Microbiology and Infection 1995 1, 127-133DOI: (10.1111/j.1469-0691.1995.tb00457.x) Copyright © 1995 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 PFGE of XbaI-digested genomic DNA of EIEC 0124. (A) Lane 1 to 7, sporadic isolates (lane 1, strain 25; lane 2, strain 26; lane 3, strain 106; lane 4, strain 139; lane 5, strain 140; lane 6, strain 141; lane 7, strain 142); lane 8, outbreak I; lane 9, outbreak II (strain 129). (B) Lane 1 to 8, outbreak II (lane 1, strain 120; lane 2, strain 122; lane 3, strain 123; lane 4, strain 135; lane 5, strain 125; lane 6, strain 128; lane 7, strain 132; lane 8, strain 136). Lanes Mi, molecular-size marker (lambda concatemer); lane M2, molecular-size marker (lambda DNA-HindIII). Clinical Microbiology and Infection 1995 1, 127-133DOI: (10.1111/j.1469-0691.1995.tb00457.x) Copyright © 1995 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 Dendrogram of the degree (%) of similarity between macrorestriction (XbaI) DNA types of of EIEC 0124 isolates. Clinical Microbiology and Infection 1995 1, 127-133DOI: (10.1111/j.1469-0691.1995.tb00457.x) Copyright © 1995 European Society of Clinical Infectious Diseases Terms and Conditions