Yaowu Xiao, Mingsheng Guo, Kevin Parker, M. Shane Hutson

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Presentation transcript:

Wavelength-Dependent Collagen Fragmentation during Mid-IR Laser Ablation Yaowu Xiao, Mingsheng Guo, Kevin Parker, M. Shane Hutson Biophysical Journal Volume 91, Issue 4, Pages 1424-1432 (August 2006) DOI: 10.1529/biophysj.106.084616 Copyright © 2006 The Biophysical Society Terms and Conditions

Figure 1 FTIR spectra of the debris collected during laser ablation of cornea. (A) A set of five independent spectra (gray), as well as their average (black), are shown for each exposure condition. Spectra from an unexposed cornea are shown for comparison. All samples were vacuum dried on CaF2 windows. Frequency ranges characteristic of isocyanate, nitrile, and alkyne groups are marked with the gray bars. (B) Difference spectra of the average debris spectrum for each condition minus the spectrum of an unexposed cornea. These difference spectra highlight the observed bands of new chemical species. Biophysical Journal 2006 91, 1424-1432DOI: (10.1529/biophysj.106.084616) Copyright © 2006 The Biophysical Society Terms and Conditions

Figure 2 Micro-Raman spectra of the debris collected during laser ablation of cornea. Each spectrum was collected from a single dried droplet of debris on a CaF2 window. The droplet corresponding to each spectrum is marked with a thin pointer line. A spectrum from an unexposed cornea is shown for comparison. Frequency ranges characteristic of isocyanate, nitrile, and alkyne groups are marked with the gray bars. Biophysical Journal 2006 91, 1424-1432DOI: (10.1529/biophysj.106.084616) Copyright © 2006 The Biophysical Society Terms and Conditions

Figure 3 13C-NMR spectra of purified collagen and the debris from high-fluence ablation of cornea at 6.45μm. The chemical shift ranges characteristic of isocyanate, nitrile, and alkyne groups are marked with the gray bars. Biophysical Journal 2006 91, 1424-1432DOI: (10.1529/biophysj.106.084616) Copyright © 2006 The Biophysical Society Terms and Conditions

Figure 4 Representative SDS-PAGE gel comparing detergent extracts of native cornea to the ablation debris collected at high fluence and either 6.45 or 2.77μm. Identifiable bands are denoted down the right-hand side. The leftmost lane contains molecular weight markers. Biophysical Journal 2006 91, 1424-1432DOI: (10.1529/biophysj.106.084616) Copyright © 2006 The Biophysical Society Terms and Conditions

Figure 5 FTIR absorbance spectra of thin slices of dehydrated cornea heated in air to temperatures between 200–500°C for 2min. Although a 2218cm−1 band does grow in as the temperature is increased above 350°C, it is accompanied by numerous spectral changes in the amide I, II, and III regions (1000–1700cm−1). The scale bar applies to absorbance units for all spectra. Biophysical Journal 2006 91, 1424-1432DOI: (10.1529/biophysj.106.084616) Copyright © 2006 The Biophysical Society Terms and Conditions