Rapid Allelic Discrimination by TaqMan PCR for the Detection of the Gilbert's Syndrome Marker UGT1A1*28 Ursula Ehmer, Tim O. Lankisch, Thomas J. Erichsen,

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Rapid Allelic Discrimination by TaqMan PCR for the Detection of the Gilbert's Syndrome Marker UGT1A1*28 Ursula Ehmer, Tim O. Lankisch, Thomas J. Erichsen, Sandra Kalthoff, Nicole Freiberg, Michael Wehmeier, Michael P. Manns, Christian P. Strassburg The Journal of Molecular Diagnostics Volume 10, Issue 6, Pages 549-552 (November 2008) DOI: 10.2353/jmoldx.2008.080036 Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Typical example of the allelic discrimination of the UGT1A1*28 variant by TaqMan PCR. NTC, no template control. The Journal of Molecular Diagnostics 2008 10, 549-552DOI: (10.2353/jmoldx.2008.080036) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Analysis of 10 samples each of one wild-type, one heterozygous, and one homozygous probe shows low interprobe variability. Mean values for VIC and FAM fluorescence are shown for each probe with bars representing mean SD. The Journal of Molecular Diagnostics 2008 10, 549-552DOI: (10.2353/jmoldx.2008.080036) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions