Actin Fringe Is Correlated with Tip Growth Velocity of Pollen Tubes Huaijian Dong, Weike Pei, Ren Haiyun  Molecular Plant  Volume 5, Issue 5, Pages 1160-1162 (September 2012) DOI: 10.1093/mp/sss073 Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

Figure 1 The Correlation between Actin Fringe and Growth Velocity of Pollen Tubes. (A–C) Representative confocal images of apical actin organization in different lily pollen tubes. The apical actin filaments were divided into three types (Types A–C) after staining with Alexa 488-phalloidin. The arrows indicate the fluorescence signals (white arrows) and fluorescence values (block arrows) in corresponding positions. (D) The average lengths of pollen tubes growing for 3h with different types of apical actin filaments (n>50). (E) The average growth velocities of pollen tubes incubated for 1.5 and 3h (n>50). (F) The percents of different types of apical actin filaments in pollen tubes growing for 1.5 and 3h (n>30). (G) Tip-localized actin fringe oscillates with pollen tube growth. (H) Representative confocal images of the tilting lily pollen tube transiently expressing Lifeact–mEGFP. The arrow represents the growth orientation of the pollen tube. (I, J) Quantitative analysis of the average fluorescence intensity of actin fringe on the both sides of untilting pollen tubes (I) and tilting pollen tubes (J) (n>30). (K) The fluorescence intensity ratio of actin fringe between faster growth side and slower growth side. Compared with untitling pollen tubes, the ratio is significantly bigger in titling pollen tubes (n>30). (L) The average growth velocity of pollen tubes incubated at 28°C is significantly higher than that of pollen tubes incubated at 16°C (n>60). (M) Representative actin fringes from lily pollen tubes cultured at a higher temperature of 28°C and a lower temperature of 16°C. The arrows show the length of the actin fringe. (N) The average length of the actin fringe in pollen tubes incubated at 28°C is significantly shorter than that of those in pollen tubes incubated at 16°C (n>30). Error bars indicate mean ± SE. ** indicates P<0.01. Bar=10μm. Molecular Plant 2012 5, 1160-1162DOI: (10.1093/mp/sss073) Copyright © 2012 The Authors. All rights reserved. Terms and Conditions