Lecture 13: Epididymis, Ejaculation and Semen Animal Science 434 Lecture 13: Epididymis, Ejaculation and Semen

Sperm Structure Head Neck Middle Piece Annulus Principal Piece Acrosome Neck Middle Piece Annulus Principal Piece End Piece

Sperm From Different Species

Sperm Head (Equatorial Segment) Nucleus Nucleus

Acrosome Plasma Membrane Nucleus

Acrosin Hyaluronidase Acrosome Nucleus Plasma Membrane

Laminated columns (flexibility) Posterior head Capitulum Laminated columns (flexibility) Mitochondrial helix (energy) Middle piece Axoneme (9 + 9 + 2) Course outer fibers

Fibrous Sheath or helix Annulus Midpiece Principal Piece Fibrous Sheath or helix

Axoneme Middle Piece Principal Piece

Axoneme

Microtubule Sliding in Sperm Tail

Sperm Metabolism Glucose Hexokinase Glucose-6-phosphate Anerobic phosphatase Hexokinase Glucose-6-phosphate Glucose Phosphohexoisomerase Anerobic Metabolism (Glycolysis) Fructose-6-phosphate Fructose Hexokinase Lactate + 2 ATP + 2 H2O Fatty Acids Aerobic Metabolism Tricarboxcylic Acid Cycle 36 ATP/Glucose 36 ATP/Fructose

ATP Utilization in Sperm motility (60%) substrate cycling (40%) wasted maintenance of ionic gradients very small amount transcription and translation none after condensation of nucleus and loss of residual body

Sperm Metabolism Temperature dependent ATP production increases as temperature increases ATP dependent processes are temperature dependent motility increases with increasing temperature

Effect of Temperature on Motility Velocity (µm/s) Temperature (C)

Transport of Sperm Vas Efferentia fluid flow cilia Caput contractions Deferens Rete Testis contractions fluid flow RT secretion Corpus contractions Seminiferous Tubule bulk fluid flow contractions of of myoid cells Cauda contractions

Epididymal Function Maturation Concentration change in fertility develop motility nuclear condensation cytoplasmic droplet Concentration remove water

Epididymal Function Secretion Transport Storage T DHT energy substrates (GPC, carnitine) glycoproteins, lipids, enzymes Transport smooth muscle contraction Storage cauda

Hormonal Control of Epididymis Caput tubal testosterone Corpus tubual dihydrotestosterone (DHT) vascular testosterone Cauda tubal DHT

Erection Sensory Psychic Sympathetic relaxation of penile retractor muscle Parasympathetic contraction of ischiocavernosus muscle dilation of vessels Inhibit vasoconstriction engorgement of corpus cavernosum decreased venous outflow

Emission and Ejaculation Sensory Psychic Parasympathetic Sympathetic smooth muscle contraction of prostate, seminal vesicles, vas deferens, cauda epididymis, closure of the sphincter to the bladder striated muscle contraction of ischiocavernosus, bulbospongiosus, pelvic urethral emission ejaculation

Ejaculate Characteristics Species Time lapse for Composition of Bull 1 second single fraction Ram 1 second single fraction Boar 5 - 25 minutes fractionated sperm-free sperm-rich coagulum Stallion 30 - 60 seconds fractionated sperm-free sperm-rich mucus Human 10 - 30 seconds single fraction but coagulated

Components of Semen water sperm substrates inorganic salts proteins fructose sorbitol inositol glycerol phosphatidyl choline (GPC) citric acid inorganic salts sodium, potassium, calcium, magnesium, chloride proteins

Initial Semen Evaluation color volume concentration motility viability morphology

Ejaculate Characteristics Species Ejaculate Sperm Total Sperm % % Volume Conc. (x109) Motile Normal (x109) /Ejaculate Bull 8.0 1.5 12 75 95 Ram 1.0 3.0 3 95 95 Boar 200 .25 50 70 90 Stallion 80 .15 12 70 40-90 Man 2-6 .15 .9 65 30-70