Expression of transgenic pum is anticonvulsive.

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Expression of transgenic pum is anticonvulsive. Expression of transgenic pum is anticonvulsive. (A) Expression of transgenic full-length pum lacking NRE motifs (UAS) in cholinergic neurons in parabss [Cha-Gal4(19B)>pum] is sufficient to reduce recovery time (RT) from mechanical shock-induced seizure in adult flies compared with parabss alone (CTRL). By contrast, further reduction of pum, through RNAi-mediated knockdown (RNAi) [Cha-Gal4(19B)>RNAi], significantly lengthens seizure RT. Each manipulation tested 10 flies per vial to produce an average value. This was repeated in triplicate and a final average calculated. Data are means±s.d., n=3. (B) Identical manipulation of pum expression in cholinergic neurons in L3 parabss had an identical effect on seizure duration when seizure was evoked by electroshock. (C) Pan-neuronal manipulation of pum is also sufficient to affect electroshock-induced seizure in L3. Upregulation (UAS: parabss;; elaV-Gal4>pum) reduces seizure and downregulation (RNAi: parabss;; elaV-Gal4>RNAi) increases seizure duration compared with control (parabss;; elaV-Gal4/+). (D) Up- or downregulation of pum in a WT background, using the Cha-Gal4(19B) cholinergic driver line, results in induction of a seizure phenotype. Data are means±s.d., n is stated in individual bars. *P≤0.05, **P≤0.01, ***P≤0.001 (two-way ANOVA with Bonferroni's post hoc). Wei-Hsiang Lin et al. Dis. Model. Mech. 2017;10:141-150 © 2017. Published by The Company of Biologists Ltd