Mara Curaba, M. Sc. , Jonathan Poels, M. Sc

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Presentation transcript:

Can prepubertal human testicular tissue be cryopreserved by vitrification? Mara Curaba, M.Sc., Jonathan Poels, M.Sc., Anne van Langendonckt, Ph.D., Jacques Donnez, M.D., Ph.D., Christine Wyns, M.D., Ph.D. Fertility and Sterility Volume 95, Issue 6, Pages 2123.e9-2123.e12 (May 2011) DOI: 10.1016/j.fertnstert.2011.01.014 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Histologic appearance of (A, B, C) fresh, (D, E, F) slow-frozen/thawed, and (G, H, I) vitrified/warmed testicular tissue from a 6-year-old boy after 10 days of in vitro organotypic culture and (A, D, G) hematoxylin-eosin (HE) staining or (B, E, H) immunohistochemical (IHC) MAGE-A4 and (C, F, I) Ki67 staining. Spermatogonial cells were identified on HE sections by their localization on the basement membrane, high nucleus-to-cytoplasm ratio, and white cytoplasmic halo around the nucleus (black arrow). Their survival was confirmed by IHC MAGE-A4 expression (black arrowhead) and their proliferative ability by Ki67 expression (open arrow). Original magnification ×200, scale bar 50 μm. Fertility and Sterility 2011 95, 2123.e9-2123.e12DOI: (10.1016/j.fertnstert.2011.01.014) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions